Cloning of a human parvovirus by molecular screening of respiratory tract samples
Cloning of a human parvovirus by molecular screening of respiratory tract samples
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DOI：
10.1073/pnas.0504666102
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发表时间：
2005-09-06
影响因子：
11.1
通讯作者：
Andersson, B
中科院分区：
文献类型：
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作者：
Allander, T;Tammi, MT;Andersson, B
新病毒种的鉴定是传染病研究的关键问题，但技术难度很大。我们开发了一种基于宿主 DNA 去除、随机 PCR 扩增、大规模测序和生物信息学的临床样本大规模分子病毒筛查系统。该技术应用于汇集的人类呼吸道样本。第一个实验在没有使用任何特定试剂的情况下检测到了七种人类病毒。检测到的病毒包括一种冠状病毒和一种细小病毒，这两种病毒当时尚无特征。细小病毒暂时命名为人类博卡病毒，在一项回顾性临床研究中在另外 17 名患者中检测到，与儿童下呼吸道感染有关。分子病毒筛选程序为检测单个或混合样品中未知病毒种类的问题提供了一种通用的独立于培养物的解决方案。我们建议可以开始对感染人类的病毒（“人类病毒组”）进行系统探索。
The identification of new virus species is a key issue for the study of infectious disease but is technically very difficult. We developed a system for large-scale molecular virus screening of clinical samples based on host DNA depletion, random PCR amplification, large-scale sequencing, and bioinformatics. The technology was applied to pooled human respiratory tract samples. The first experiments detected seven human virus species without the use of any specific reagent. Among the detected viruses were one coronavirus and one parvovirus, both of which were at that time uncharacterized. The parvovirus, provisionally named human bocavirus, was in a retrospective clinical study detected in 17 additional patients and associated with lower respiratory tract infections in children. The molecular virus screening procedure provides a general culture-independent solution to the problem of detecting unknown virus species in single or pooled samples. We suggest that a systematic exploration of the viruses that infect humans, "the human virome," can be initiated.