Application of tissue engineering techniques for rotator cuff regeneration using a chitosan-based hyaluronan hybrid fiber scaffold
Application of tissue engineering techniques for rotator cuff regeneration using a chitosan-based hyaluronan hybrid fiber scaffold
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DOI：
10.1177/0363546504272689
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发表时间：
2005-08-01
影响因子：
4.8
通讯作者：
Nishimura, S
中科院分区：
文献类型：
--
作者：
Funakoshi, T;Majima, T;Nishimura, S
背景：目前的外科手术治疗不可修复的肩袖撕裂有相当大的局限性。使用新型支架材料的组织工程技术为治疗这些疾病提供了潜在的替代方案。假设：壳聚糖基透明质酸混合支架可以增强I型胶原蛋白与种子成纤维细胞的产物，从而提高体内再生肌腱的机械强度。研究设计：实验室对照研究。方法：采用壳聚糖基透明质酸杂化聚合物纤维制备支架。48根兔冈下肌腱及其肱骨插入处被切除以形成缺损。每个缺损用成纤维细胞种子支架（n = 16）或非成纤维细胞种子支架（n = 16）覆盖。在其他16个肩部中，肩袖缺损作为对照。在术后4周和12周，通过组织学、免疫组织化学（n = 2）和生物力学（n = 6）分析对工程肌腱进行评估。结果：I型胶原蛋白仅在成纤维细胞种子支架中可见，在再生组织中增加。术后4 ~ 12周，成纤维细胞种子支架的抗拉强度和切线模量均有明显改善。在12周时，成纤维细胞种子支架的切线模量明显高于非成纤维细胞种子支架和对照组。结论：该支架材料促进了体内I型胶原蛋白的生成，提高了肌腱套再生组织的机械强度。临床意义：使用这种组织工程技术进行肩袖再生是可行的。
Background: The current surgical procedures for irreparable rotator cuff tears have considerable limitations. Tissue engineering techniques using novel scaffold materials offer potential alternatives for managing these conditions.Hypothesis: A chitosan-based hyaluronan hybrid scaffold could enhance type I collagen products with seeded fibroblasts and thereby increase the mechanical strength of regenerated tendon in vivo.Study Design: Controlled laboratory study.Methods: The scaffolds were created from chitosan-based hyaluronan hybrid polymer fibers. Forty-eight rabbit infraspinatus tendons and their humeral insertions were removed to create defects. Each defect was covered with a fibroblast-seeded scaffold (n = 16) or a non-fibroblast-seeded scaffold (n = 16). In the other 16 shoulders, the rotator cuff defect was left free as the control. At 4 and 12 weeks after surgery, the engineered tendons were assessed by histological, immunohistochemical (n = 2), and biomechanical (n = 6) analyses.Results: Type I collagen was only seen in the fibroblast-seeded scaffold and increased in the regenerated tissue. The tensile strength and tangent modulus in the fibroblast-seeded scaffold were significantly improved from 4 to 12 weeks postoperatively. The fibroblast-seeded scaffold had a significantly greater tangent modulus than did the non-fibroblast-seeded scaffold and the control at 12 weeks.Conclusion: This scaffold material enhanced the production of type I collagen and led to improved mechanical strength in the regenerated tissues of the rotator cuff in vivo.Clinical Relevance: Rotator cuff regeneration is feasible using this tissue engineering technique.