STUDY OF FIELD-GROWN COTTON ROOTS INFECTED WITH VERTICILLIUM-DAHLIAE USING AN IMMUNOENZYMATIC STAINING TECHNIQUE
STUDY OF FIELD-GROWN COTTON ROOTS INFECTED WITH VERTICILLIUM-DAHLIAE USING AN IMMUNOENZYMATIC STAINING TECHNIQUE
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DOI：
10.1094/phyto-78-1174
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发表时间：
1988-09-01
期刊：
影响因子：
3.2
通讯作者：
HUISMAN, OC
中科院分区：
文献类型：
--
作者：
GERIK, JS;HUISMAN, OC
采用特异性免疫酶染色技术研究了棉花黄萎病菌在大田棉花根系中的定殖。大丽轮枝菌的菌落密度随距离根尖的增加而增加，最大密度出现在距根尖1cm以上。在距离尖端超过1厘米的菌落密度相对恒定。大丽轮枝菌的平均菌落长度为7.3mm，菌落长度的增加与距根尖的距离有关。大丽轮枝菌的菌丝遍布整个皮层，在维管柱表面的根皮层内部最多。殖民地外观与菌丝从根表面向中柱生长一致。大丽轮枝菌的菌丝也在许多皮层细胞中被发现。尖孢镰刀菌的殖民地，同样染色，被发现主要局限于根表面和外皮层。
Colonization of field-grown cotton roots by Verticillium dahliae was studied by using a specific immunoenzymatic staining technique. Colony density of V. dahliae increased with distance from the root tip, with the maximal density occurring more than 1 cm from the root apex. Colony densities at distances more than 1 cm from the tip were relatiely constant. The mean colony length of V. dahliae was 7.3 mm, and increased colony length was correlated with distance from root apices. Hyphae of V. dahliae were present through the entire depth of the cortex, and were greatest in the interior of the root cortex at the surface of the vascular cylinder. The colony appearance was consistent with growth of hyphae from the root surface toward the stele. Hyphae of V. dahliae also were found within numerous cortical cells. Colonies of Fusarium oxysporum, similarly stained, were found to be mostly confined to the root surface and the outer cortex.