Feasibility of ultra-performance liquid chromatography-ion mobility-time-of-flight mass spectrometry in analyzing oxysterols.
Feasibility of ultra-performance liquid chromatography-ion mobility-time-of-flight mass spectrometry in analyzing oxysterols.
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DOI：
10.1016/j.chroma.2017.01.039
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发表时间：
2017-03
期刊：
影响因子：
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通讯作者：
Petri Kylli;T. Hankemeier;R. Kostiainen
中科院分区：
文献类型：
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作者：
Petri Kylli;T. Hankemeier;R. Kostiainen
氧甾醇是含氧胆固醇，对许多细胞功能很重要，也可能预示某些疾病。本工作的目的是研究使用行波池的超高效液相色谱-离子淌度-飞行时间质谱（UPLC-IM-TOFMS）分析生物样品中的氧甾醇，特别是其异构体的可行性。氧甾醇作为对甲苯磺酰基异氰酸酯衍生物进行分析，通过电喷雾电离步骤中的离子淌度和电离效率改善了异构氧甾醇的分离。 UPLC-IM-TOFMS 方法速度快，定量性能良好。该方法的可行性在成纤维细胞样品中氧甾醇的分析中得到了证明。
Oxysterols are oxygenated cholesterols that are important in many cell functions and they may also be indicative of certain diseases. The purpose of this work was to study the feasibility of ultra-performance liquid chromatography–ion mobility–time-of-flight mass spectrometry (UPLC–IM–TOFMS) using traveling wave cell in analyzing oxysterols and especially their isomers in biological samples. Oxysterols were analyzed as theirp-toluenesulfonyl isocyanate derivatives, which improved the separation of isomeric oxysterols by ion mobility and ionization efficiency in the electrospray ionization step. The UPLC–IM–TOFMS method was shown to be fast and to provide good quantitative performance. The feasibility of the method was demonstrated in the analyses of oxysterols in fibroblast cell samples.