Identification of a novel proliferation-related protein, WHSC1 4a, in human gliomas
Identification of a novel proliferation-related protein, WHSC1 4a, in human gliomas
复制标题
DOI：
10.1215/15228517-2007-036
复制
发表时间：
2008-02-01
期刊：
影响因子：
15.9
通讯作者：
Zhuang, Zhengping
中科院分区：
文献类型：
--
作者：
Li, Jie;Yin, Chunyue;Zhuang, Zhengping
多个基因表达的动态变化似乎是区分转化细胞与正常细胞的共同特征。我们比较了四种多形性胶质母细胞瘤 (GBM) 组织样本和四种正常大脑皮层样本的蛋白质组谱，以检查神经胶质瘤发生的分子基础。胰蛋白酶消化的蛋白质样品通过毛细管等电聚焦和纳米反相液相色谱进行分离，并通过质谱测序进行分析。 Wolf-Hirschhorn 综合征候选 1 (WHSC1) 以及其他 103 种蛋白质仅在 GBM 蛋白质组中发现。蛋白质印迹和免疫组织化学验证了我们的蛋白质组学发现，并证明 30-kDa WHSC1 表达随着肿瘤增殖活性的上升而增加。 RNA 干扰可以通过阻断 WHSC1 表达来抑制神经胶质瘤细胞的生长。我们的新发现鼓励蛋白质组技术在癌症研究中的应用。
Dynamic changes in the expression of multiple genes appear to be common features that distinguish transformed cells from their normal counterparts. We compared the proteomic profiles of four glioblastoma multiforme (GBM) tissue samples and four normal brain cortex samples to examine the molecular basis of gliomagenesis. Trypsin-digested protein samples were separated by capillary isoelectric focusing with nano-reversed-phase liquid chromatography and were profiled by mass spectrometric sequencing. Wolf-Hirschhorn syndrome candidate 1 (WHSC1), along with 103 other proteins, was found only in the GBM proteomes. Western blot and immunohistochemistry verified our proteomic findings and demonstrated that 30-kDa WHSC1 expression increases with ascending tumor proliferation activity. RNA interference could suppress glioma cell growth by blocking WHSC1 expression. Our novel findings encourage the application of proteomic techniques in cancer research.