Endothetial progenitor cells contribute to accelerated liver regeneration
Endothetial progenitor cells contribute to accelerated liver regeneration
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DOI：
10.1016/j.jpedsurg.2007.02.034
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发表时间：
2007-07-01
影响因子：
2.4
通讯作者：
Puder, Mark
中科院分区：
文献类型：
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作者：
Beaudry, Pau;Hida, Yasuhiro;Puder, Mark
循环内皮细胞（circulating endothelial cells，CEC）分为两类，一类来源于骨髓，另一类来源于骨髓，干细胞标记物CD117或CD133与内皮细胞特异性抗原的表达相结合，被称为循环内皮祖细胞（circulating endothelial progenitors，CEP）。我们已经证明外源性血管内皮生长。注意因子（VEGF）有效地动员CEP细胞。此外，已经证明VEGF调节部分肝切除术后的肝再生。虽然局部内皮细胞可以在肝再生过程中调节组织质量，但CEPs对这一过程的贡献尚不清楚。我们发现CD117和CD133从小鼠CEP细胞的损失，这两个标志物低估了骨髓来源的CEP细胞的数量。因此，我们使用野生型和绿色荧光蛋白（GFP）骨髓移植到野生型小鼠，并进行70%的肝切除术。此外，我们发现，治疗与外源性VEGF加速肝再生后，70%的肝切除术，而免疫组化分析显示，7倍的CEP细胞纳入肝血管。这些结果表明，CEP细胞在调节肝再生中起作用，并且VEGF处理可以动员CEP细胞来加速该过程。(c)2007年爱思唯尔公司All rights reserved.
Two classes of circulating endothelial cells (CECs) have been identified and are distinguished by the expression of the stem cell markers CD117 or CD133 together with endothelial-specific antigens.Stem cell marker-positive CECs originate from bone marrow and have been designated as circulating endothelial progenitors (CEPs). We have demonstrated that exogenous vascular endothelial growth. note factor (VEGF) effectively mobilizes CEP cells. Furthermore, it has been demonstrated that VEGF regulates liver regeneration after partial hepatectomy. Although local endothelial cells can regulate tissue mass during liver regeneration, the contribution of CEPs to this process is unknown. We discovered loss of CD117 and CD133 from murine CEP cells and that both markers underestimated the number of bone marrow-derived CEP cells. We therefore used wild type and green fluorescent protein (GFP)-bone marrow transplanted into wild-type mice and performed 70% hepatectomies. Furthermore, we found that treatment with exogenous VEGF accelerated liver regeneration after 70% hepatectomy, whereas immunohistochemical analysis showed a 7-fold increase in the incorporation of CEP cells into liver vasculature. These results suggest that CEP cells play a role in regulating liver regeneration and that VEGF treatment can mobilize CEP cells to accelerate this process. (c) 2007 Elsevier Inc. All rights reserved.