Coordinate control of syntheses of ribosomal ribonucleic acid and ribosomal proteins during nutritional shift-up in Saccharomyces cerevisiae
Coordinate control of syntheses of ribosomal ribonucleic acid and ribosomal proteins during nutritional shift-up in Saccharomyces cerevisiae
复制标题
DOI：
10.1128/mcb.1.11.1007-1015.1981
复制
发表时间：
1981-11
影响因子：
5.3
通讯作者：
D. R. Kief;J. R. Warner
中科院分区：
文献类型：
--
作者：
D. R. Kief;J. R. Warner
我们研究了核糖体合成的调节酿酒酵母生长在不同的速度和响应于生长刺激。核糖体含量和核糖体核糖核酸和核糖体蛋白质的合成速率进行了比较，在基本培养基中生长的葡萄糖或乙醇作为碳源的文化。结果表明，核糖体含量与生长速率成正比。此外，这些稳态浓度在核糖体前体核糖核酸和核糖体蛋白质的合成水平上受到调节。当在乙醇上生长的培养物富含葡萄糖时，通过用[甲基-3H]甲硫氨酸脉冲细胞测量的核糖体核糖核酸合成速率在5分钟内增加了40%，在15分钟内增加了一倍，并在30 min时达到新生长培养基的稳态特征。亮氨酸显示与总细胞蛋白质的合成速率相比，30种或更多核糖体蛋白质的合成速率协调增加。它们的合成在15分钟内刺激约2.5倍，在60分钟内刺激近4倍。酿酒酵母通过优先刺激核糖体核糖核酸和核糖体蛋白质的合成来响应生长刺激。
We investigated the regulation of ribosome synthesis in Saccharomyces cerevisiae growing at different rates and in response to a growth stimulus. The ribosome content and the rates of synthesis of ribosomal ribonucleic acid and of ribosomal proteins were compared in cultures growing in minimal medium with either glucose or ethanol as a carbon source. The results demonstrated that ribosome content is proportional to growth rate. Moreover, these steady-state concentrations are regulated at the level of synthesis of ribosomal precursor ribonucleic acid and of ribosomal proteins. When cultures growing on ethanol were enriched with glucose, the rate of ribosomal ribonucleic acid synthesis, measured by pulsing cells with [methyl-3H]methionine, increased by 40% within 5 min, doubled within 15 min, and reached a steady state characteristic of the new growth medium by 30 min. Labeling with [3H]leucine reveal a coordinate increase in the rate of synthesis of 30 or more ribosomal proteins as compared with that of total cellular proteins. Their synthesis was stimulated approximately 2.5-fold within 15 min and nearly 4-fold within 60 min. The data suggest that S. cerevisiae responds to a growth stimulus by preferential stimulation of the synthesis of ribosomal ribonucleic acid and ribosomal proteins.