PHORBOL DIESTER-INDUCED MACROPHAGE DIFFERENTIATION OF LEUKEMIC BLASTS FROM PATIENTS WITH HUMAN MYELOGENOUS LEUKEMIA
PHORBOL DIESTER-INDUCED MACROPHAGE DIFFERENTIATION OF LEUKEMIC BLASTS FROM PATIENTS WITH HUMAN MYELOGENOUS LEUKEMIA
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DOI：
10.1172/jci109939
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发表时间：
1980-01-01
影响因子：
15.9
通讯作者：
TERRITO, M
中科院分区：
文献类型：
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作者：
KOEFFLER, HP;BARELI, M;TERRITO, M
佛波酯，包括12-O-四-卡诺酰基佛波13-乙酸酯（TPA），诱导人急性髓性白血病细胞系的终末巨噬细胞样分化。从患者获得的髓性白血病细胞在暴露于TPA后经历巨噬细胞样分化。用TPA培养的髓性白血病细胞变得粘附于具有长丝状伪足的带电表面，对α-SMA产生阳性染色。乙酸萘酯酶，增加溶菌酶分泌，减少硝基四氮唑蓝和获得吞噬念珠菌的能力。当用TPA培养时，来自淋巴细胞白血病患者的细胞没有变成巨噬细胞样。
Phorbol esters, including 12-O-tetrade-canoylphorbol 13-acetate (TPA), induce terminal macrophage-like differentiation of cells from human acute myelogenous leukemia lines. Myelogenous leukemia cells obtained from patients undergo macrophage-like differentiation after exposure to TPA. The myeloid leukemic cell cultured with TPA became adherent to charged surfaces with long filamentous pseudopodia, developed positive staining for .alpha.-napthyl acetate esterase, increased lysozyme secretion, reduced nitroblue tetrazolium and acquired the ability to phagocytose Candida. Cells from patients with lymphocytic leukemia did not become macrophage-like when cultured with TPA.