Alterations in the epithelial surface of A/Jax mouse palatal shelves prior to and during palatal fusion: A scanning electron microscopic study
Alterations in the epithelial surface of A/Jax mouse palatal shelves prior to and during palatal fusion: A scanning electron microscopic study
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A/Jax 小鼠腭架在腭融合之前和过程中上皮表面的变化：扫描电子显微镜研究
DOI：
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发表时间：
1973
期刊：
影响因子：
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通讯作者：
S. Meller
中科院分区：
文献类型：
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作者：
R. Waterman;L. Ross;S. Meller
在腭融合之前和过程中，用扫描电子显微镜检查了精心分期的 A/Jax 小鼠胚胎的腭架表面。覆盖架子的口腔和鼻表面的细胞的外观没有观察到变化。然而，在推定融合区域观察到了变化，并且在转位之前可以在垂直架子上检测到。这种变化最初涉及沿着第二至第三褶皱水平的未来架子内侧边缘的表面细胞。其特征是明显的细胞边界丧失、相邻细胞明显收缩引起的细胞间间隙的出现以及表面丝状物质的逐渐积累。在转位和融合过程中，这些改变沿着架子的内侧边缘向后传播。虽然不可能从 S.E.M. 确定表面变化的精确性质。仅凭数据来看，它既发生在闭合之前，又仅限于架子之间随后接触和融合的区域，这一事实表明，它可能是正常腭融合的先决条件，并且可能代表获得融合潜力的表现，类似于体外腭融合所提出的融合潜力。
The surfaces of palatal shelves from carefully staged A/Jax mouse embryos were examined with the scanning electron microscope prior to and during palatal fusion. No change was observed in the appearance of cells covering the oral and nasal surfaces of the shelves. A change was observed, however, in the region of presumptive fusion, and was detectable on vertical shelves just prior to transposition. This change initially involved surface cells along the future medial edge of the shelf at the level of the second to third rugae. It was characterized by a loss of distinct cellular boundaries, the appearance of intercellular gaps caused by apparent retraction of adjacent cells, and a progressive accumulation of filamentous material at the surface. These alterations spread posteriorly along the medial edge of the shelf during transposition and fusion. While it was impossible to determine the precise nature of the surface change from S.E.M. data alone, the fact that it both preceded closure and was restricted to the region of subsequent contact and fusion between the shelves suggests it may be a prerequisite for normal palatal fusion and may represent an expression of the acquisition of a potential to fuse similar to that proposed for palatal fusion in vitro.