A NEW FAMILY OF GROWTH FACTOR-LIKE PEPTIDES - TREFOIL DISULFIDE LOOP STRUCTURES AS A COMMON FEATURE IN BREAST-CANCER ASSOCIATED PEPTIDE (PS2), PANCREATIC SPASMOLYTIC POLYPEPTIDE (PSP), AND FROG-SKIN PEPTIDES (SPASMOLYSINS)
A NEW FAMILY OF GROWTH FACTOR-LIKE PEPTIDES - TREFOIL DISULFIDE LOOP STRUCTURES AS A COMMON FEATURE IN BREAST-CANCER ASSOCIATED PEPTIDE (PS2), PANCREATIC SPASMOLYTIC POLYPEPTIDE (PSP), AND FROG-SKIN PEPTIDES (SPASMOLYSINS)
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DOI：
10.1016/0014-5793(89)80690-8
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发表时间：
1989-06-19
期刊：
影响因子：
3.5
通讯作者：
THIM, L
中科院分区：
文献类型：
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作者：
THIM, L
存在于完全不同的生物来源中的四种肽已显示出很大程度的结构相似性。所述肽包括：（i）从人胃液和人乳腺癌细胞系MCF-7的培养基中分离的60个氨基酸残基的乳腺癌相关pS2肽;（ii）从猪胰腺和胰液中分离的106个氨基酸残基的胰腺痉挛多肽（PSP）;和（iii）从分离自蛙非洲爪蟾皮肤的cDNA预测的49和50个氨基酸残基的肽。这些肽的特征在于具有一个（pS2和青蛙肽）或两个（PSP）高度保守的38-39个氨基酸残基共有序列的结构域，该序列在任何其它已知的肽或蛋白质中未发现。结构域序列在几乎相同的位置含有6个半胱氨酸残基，并且表明这6个残基通过3个二硫键连接以形成在所有四种肽中常见的特征性“三叶”二硫环结构。从已经分离的肽的来源和显示PSP对MCF-7细胞具有生长因子刺激作用的实验，进一步表明这些肽可能代表新的生长因子家族的成员。
Four peptides present in completely different biological sources have been shown to exhibit a large degree of structural similarity. The peptides include: (i) a 60 amino acid residue breast cancer associated pS2 peptide isolated from human gastric juice and the culture media of the human breast cancer cell line MCF-7; (ii) a 106 amino acid residue pancreatic spasmolytic polypeptide (PSP) isolated from porcine pancreas and pancreatic juice; and (iii) a 49 and 50 amino acid residue peptide predicted from a cDNA isolated from the skin of the frog,Xenopus laevis. These peptides are characterized by having one (pS2 and the frog peptides) or two (PSP) domains of a highly conserved 38–39 amino acid residue consensus sequence not found in any other known peptides or proteins. The domain sequences contain 6 cysteine residues in nearly the same positions and it is suggested that these 6 residues are linked by 3 disulphide bonds to form a characteristic ‘trefoil’ disulphide loop structure common in all four peptides. From the sources of which the peptides have been isolated and from experiments showing that PSP has a growth factor stimulatory effect on MCF-7 cells, it is further suggested that these peptides may represent members of a new family of growth factors.