Organic Solvent-Free Fluorescence Confocal Imaging of Living Cells Using Pure Nanocrystal Forms of Fluorescent Dyes
Organic Solvent-Free Fluorescence Confocal Imaging of Living Cells Using Pure Nanocrystal Forms of Fluorescent Dyes
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DOI：
10.1143/jjap.48.117002
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发表时间：
2009-11-01
影响因子：
1.5
通讯作者：
Nakanishi, Hachiro
中科院分区：
文献类型：
--
作者：
Baba, Koichi;Kasai, Hitoshi;Nakanishi, Hachiro
在本文中，我们描述了一种无有机溶剂的活细胞体外荧光共聚焦成像的方法，使用疏水性荧光染料3，3 '-双十八烷基氧碳菁高氯酸盐（DiO）和二萘嵌苯的纳米晶体，我们分别使用再沉淀法获得。共聚焦显微镜成像显示，癌细胞以高亲合力摄取染料。在类似的处理条件下，染料以其水溶性形式或作为二甲亚砜溶液通过共聚焦显微镜提供了相当的细胞体外荧光成像。（即，有机溶剂、表面活性剂），其通常表现出细胞毒性。我们开发的技术还允许使用DiO和二萘嵌苯纳米晶体对癌细胞进行双重染色成像（C）2009日本应用物理学会DOI：10. 1143/JJAP. 48. 117002
In this paper we describe an organic solvent-free method for the in vitro fluorescence confocal imaging of living cells using nanocrystals of the hydrophobic fluorescent dyes 3,3'-dioctadecyloxacarbocyanine perchlorato (DiO) and perylene, which we obtained separately using the reprecipitation method. Confocal microscopy imaging revealed that cancer cells took up the dye with high avidity Under similar treatment conditions, the dyes in their nanocrystal forms or as dimethylsulfoxide solutions provided comparable in vitro fluorescence imaging of cells through confocal microscopy This nanocrystal formulation approach to in vitro bioimaging in cultures eliminates the need for external solubilizing agents (i.e, organic solvent, surfactant), which usually exhibit cytotoxicity Our developed technique also allowed the double-staining imaging of cancer cells using DiO and perylene nanocrystals (C) 2009 The Japan Society of Applied Physics DOI: 10.1143/JJAP.48.117002