Construction of implantable optical fibers for long-term optogenetic manipulation of neural circuits.
Construction of implantable optical fibers for long-term optogenetic manipulation of neural circuits.
复制标题
构建可植入的光纤，用于对神经回路的长期光遗传操作。
DOI：
10.1038/nprot.2011.413
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发表时间：
2011-12-08
期刊：
影响因子：
14.8
通讯作者：
Stuber GD
中科院分区：
文献类型：
--
作者：
Sparta DR;Stamatakis AM;Phillips JL;Hovelsø N;van Zessen R;Stuber GD
In vivo optogenetic strategies have redefined our ability to assay how neural circuits govern behavior. Although acutely implanted optical fibers have previously been used in such studies, long-term control over neuronal activity has been largely unachievable. Here we describe a method to construct implantable optical fibers to readily manipulate neural circuit elements with minimal tissue damage or change in light output over time (weeks to months). Implanted optical fibers readily interface with in vivo electrophysiological arrays or electrochemical detection electrodes. The procedure described here, from implant construction to the start of behavioral experimentation, can be completed in approximately 2–6 weeks. Successful use of implantable optical fibers will allow for long-term control of mammalian neural circuits in vivo, which is integral to the study of the neurobiology of behavior.
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通讯作者：
Deisseroth, Karl
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影响因子：
14.8
作者：
通讯作者：
--
影响因子：
14.8
作者：
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通讯作者：
Deisseroth K
影响因子：
48
作者：
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通讯作者：
Ramanathan, Sharad