The flowering integrator FT regulates SEPALLATA3 and FRUITFULL accumulation in Arabidopsis leaves
The flowering integrator FT regulates SEPALLATA3 and FRUITFULL accumulation in Arabidopsis leaves
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DOI：
10.1105/tpc.105.035766
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发表时间：
2005-10-01
期刊：
影响因子：
11.6
通讯作者：
Samach, A
中科院分区：
文献类型：
--
作者：
Teper-Bamnolker, P;Samach, A
向开花的过渡涉及到茎尖分生组织和现存叶原的命运的主要变化。拟南芥（Arabidopsis thaliana）促花基因开花位点T（开花位点T， flower LOCUS T， FT）转录本存在于叶片组织中，人工导入分生组织后也能促进开花。FT通常在叶片和/或分生组织中起作用，启动或构成一个可移动的促花信号。我们研究了莲座叶中与ft相关的事件，其中一些事件可能先于或模仿分生组织及其原基中的事件。我们在叶片中发现了MADS盒转录因子FRUITFULL （FUL）和SEPALLATA3 （SEP3）的ft依赖性转录积累。异常高水平的FT进一步增加了这些基因的表达，导致叶片的形态变化。开花时间基因FD的缺失，以及延迟开花的环境条件，通过减少其靶基因的激活，降低了FT对叶片的影响。分生组织中FUL、SEP3和APETALA1的积累与开花过渡有关。我们认为，FT通过这些和未知基因的伴侣依赖性转录激活起作用，并且这种激活发生在几个位点。器官的命运可能取决于激活的程度和器官在激活发生前所达到的发育阶段。
The transition to flowering involves major changes in the shoot apical meristem and in the fate of existing leaf primordia. Transcripts of the Arabidopsis thaliana flowering-promoting gene FLOWERING LOCUS T (FT) are present in leaf tissue but can also promote flowering when artificially introduced into the meristem. FT may normally act in the leaf and/or the meristem, initiating or constituting a mobile flower-promoting signal. We studied FT-dependent events in the rosette leaf, some of which might precede or mimic events in the meristem and its primordia. We show FT-dependent transcript accumulation of the MADS box transcription factors FRUITFULL (FUL) and SEPALLATA3 (SEP3) in leaves. Abnormally high levels of FT further increase the expression of these genes, leading to morphological changes in the leaves. Loss of the flowering-time gene FD, as well as environmental conditions that delay flowering, reduce FT's effect on leaves via reduced activation of its targets. FUL, SEP3, and APETALA1 accumulation in the meristem is associated with and contributes to the transition to flowering. We propose that FT functions through partner-dependent transcriptional activation of these and as-yet-unknown genes and that this occurs at several sites. Organ fate may depend on both degree of activation and the developmental stage reached by the organ before activation occurs.