Gene genealogies and AFLP analyses in the Fusarium oxysporum complex identify monophyletic and nonmonophyletic formae speciales causing wilt and rot disease
Gene genealogies and AFLP analyses in the Fusarium oxysporum complex identify monophyletic and nonmonophyletic formae speciales causing wilt and rot disease
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DOI：
10.1094/phyto.2000.90.8.891
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发表时间：
2000-08-01
期刊：
影响因子：
3.2
通讯作者：
Waalwijk, C
中科院分区：
文献类型：
--
作者：
Baayen, RP;O'Donnell, K;Waalwijk, C
通过构建核基因和线粒体基因系谱以及基于扩增片段长度多态性(AFLP)的系统发育分析，验证了植物致病镰刀菌复合体中寄主专一性分类群的单系起源，这些菌株代表了与枯萎病和根球腐病相关的8个种的已知遗传和致病多样性。我们纳入了来自尖孢镰刀菌无性系的菌株。芦笋、石竹、唐葛兰、百合、荔枝、仙人掌、菠菜和郁金香。来自康乃馨和百合的推定的非致病菌株被包括在内，来自先前在尖孢镰刀菌复合体中鉴定的三个主要支系的一个参考菌株被包括在内；来自相关物种的序列被用作外群。核转录延长因子1α和线粒体小亚基(MtSSU)核糖体RNA基因的DNA序列被结合起来进行系统发育分析。营养体亲和群(VCGS)的菌株具有相同的序列和AFLP图谱，支持两个单一的VCG组种百合和郁金香的单系性。3种VCGS在尖孢镰刀菌中也存在相同的基因型别。菠菜。相反，尖孢镰刀菌f.spp具有明显的多重进化起源。芦笋、石笋、唐菖蒲、荔枝和仙人掌，尽管这些种内不同的VCG通常聚在一起或共享相同的EF-1α和mtSSU rDNA单倍型。Kishino-Hasegawa对迫使这些种的单系化的限制分析支持尖孢镰刀菌f.sp.的唯一起源。Opuniarum，而不是尖孢镰刀菌F.spp的单株。石蒜、石榴花、唐葛兰和丽尼。香石竹和百合上的大多数非致病菌株代表了独特的VCGS，与尖孢镰刀菌f.spp无关。Dianthi和Lilii分别为。推测非致病或腐烂的菌株在分子系统发育中没有形成排他性的类群。AFLP指纹数据的简约分析支持基于基因系谱的系谱；然而，基于AFLP的系谱比基因系谱的同源性要高得多。尖孢镰刀菌复合体中的特殊变种命名系统的预测价值受到质疑。
The monophyletic origin of host-specific taxa in the plant-pathogenic Fusarium oxysporum complex was tested by constructing nuclear and mitochondrial gene genealogies and amplified fragment length polymorphism (AFLP)-based phylogenies for 89 strains representing the known genetic and pathogenic diversity in 8 formae speciales associated with wilt diseases and root and bulb rot. We included strains from clonal lineages of F. oxysporun f. spp. asparagi, dianthi, gladioli, lilii, lini, opuntiarum, spinaciae, and tulipae. Putatively nonpathogenic strains from carnation and lily were included and a reference strain from each of the three main clades identified previously in the F. oxysporum complex; sequences from related species were used as outgroups. DNA sequences from the nuclear translation elongation factor 1 alpha, and the mitochondrial small subunit (mtSSU) ribosomal RNA genes were combined for phylogenetic analysis. Strains in vegetative compatibility groups (VCGs) shared identical sequences and AFLP profiles, supporting the monophyly of the two single-VCG formae speciales, lilii and tulipae. identical genotypes were also found for the three VCGs in F. oxysporum f. sp. spinaciae. In contrast, multiple evolutionary origins were apparent for F. oxysporum, f. spp. asparagi, dianthi, gladioli, lini, and opuntiarum, although different VCGs within each of these formae speciales often clustered close together or shared identical EF-1 alpha and mtSSU rDNA haplotypes. Kishino-Hasegawa analyses of constraints forcing the monophyly of these formae speciales supported the exclusive origin of F. oxysporum f. sp. opuntiarum but not the monophyly of F. oxysporum f. spp. asgaragi, dianthi, gladioli, and lini. Most of the putatively nonpathogenic strains from carnation and lily, representing unique VCGs, were unrelated to F. oxysporum f. spp. dianthi and lilii, respectively. Putatively nonpathogenic or rot-inducing strains did not form exclusive groups within the molecular phylogeny. Parsimony analyses of AFLP fingerprint data supported the gene genealogy-based phylogram; however, AFLP-based phylogenies were considerably more homoplasious than the gene genealogies. The predictive value of the forma specialis naming system within the F. oxysporum complex is questioned.