Linkage maps of microsatellite DNA markers for the pacific oyster Crassostrea gigas
Linkage maps of microsatellite DNA markers for the pacific oyster Crassostrea gigas
复制标题
DOI：
10.1534/genetics.104.027342
复制
发表时间：
2004-09-01
期刊：
影响因子：
3.3
通讯作者：
Hedgecock, D
中科院分区：
文献类型：
--
作者：
Hubert, S;Hedgecock, D
利用来自3个科的102个微卫星DNA标记，构建了太平洋牡蛎长牡蛎（Crassostrea g,gigas） 11日龄幼虫的雄性和雌性共识连锁图谱。我们分别在男性和女性共识图中确定了11个和12个连锁群。然而，对这些单独的图谱进行比对表明，有10个连锁组，这与单倍体染色体数目一致。雄性遗传图谱包含88个位点，跨度为616.1 cM；雌性遗传图谱包含86个位点，跨度为770.5 cM。雄性和雌性地图共有74个位点；两个市场仍然没有联系。基于对基因组长度的两种估计，共识连锁图谱的估计覆盖率为男性79%，女性70-75%。95%的基因组预计分别位于雄性和雌性图谱上标记的16厘米和21厘米范围内，而95%的模拟最小距离分别在10.1厘米和13.6厘米范围内。在这三个家庭的双亲共有的118对连锁标记中，女性的重组率明显高于男性。在不同家庭的同性父母和异性兄弟姐妹父母之间，标记的重组和顺序也存在显著差异。这些观察结果表明，染色体重排多态性可能存在于自然种群中，这可能对解释牡蛎的进化遗传学具有深远的意义。这是使用微卫星DNA市场的双壳类软体动物的第一个连锁图谱，这将使它们能够转移到其他科，并对进一步的遗传分析（如QTL定位）有用。
We constructed male and female consensus linkage maps for the Pacific oyster Crassostrea g,gigas, using a total of 102 microsatellite DNA markers typed in 11-day-old larvae from three families. We identified 11 and 12 linkage groups in the male and Fern ale consensus maps, respectively. Alignment of these separate maps, however, suggests 10 linkage groups, which agrees with the haploid chromosome number. The male linkage map comprises 88 loci and spans 616.1 cM, while the female map comprises 86 loci and spans 770.5 cM. The male and the female maps share 74 loci; 2 markets remain unlinked. The estimated coverages for the consensus linkage maps are 79% for the male and 70-75% for the female, on the basis of two estimates of genome length. Ninety-five percent of the genome is expected to lie within 16 and 21 cM of markers on the male and female maps, respectively, while 95% of simulated minimum distances to the male and female maps are within 10.1 and 13.6 cM, respectively. Females have significantly more recombination than males, across 118 pairs of linked markers in common to the parents of the three families. Significant differences in recombination and orders of markers are also evident among same-sex parents of different families as well as sibling parents of opposite sex. These observations Suggest that polymorphism for chromosomal rearrangements may exist in natural populations, which could have profound implications for interpreting the evolutionary genetics of the oyster. These are the first linkage maps for a bivalve mollusc that use microsatellite DNA markets, which should enable them to be transferred to other families and to be useful for further genetic analyses such as QTL mapping.