Effect of low fluence diode laser irradiation on the hydraulic conductivity of perfused trabecular meshwork endothelial cell monolayers
Effect of low fluence diode laser irradiation on the hydraulic conductivity of perfused trabecular meshwork endothelial cell monolayers
复制标题
DOI：
10.1080/02713680701486394
复制
发表时间：
2007-01-01
影响因子：
2
通讯作者：
Weber, Paul A.
中科院分区：
文献类型：
--
作者：
Roberts, Cynthia J.;Rivera, Brian K.;Weber, Paul A.
目的：旨在确定低通量二极管激光照射对培养的人小梁网细胞单层的液体灌注特性的影响，该细胞单层放置在专门设计的试验装置中，并受到流体静压梯度驱动的液体流动的影响。方法：进行两个实验系列。在第一个系列中，使用在过滤器支持物上生长的培养的人小梁网细胞单层进行六个低通量二极管激光照射实验。在达到约5.0 mmHg的稳态灌注条件后，使用二极管激光器（λ = 810 nm）以0.2619至0.8571 J/cm（2）的能量密度照射单层。灌注和数据收集在照射后持续45分钟，之后测试单层以确定实验后活力。以2.5分钟的间隔分析了辐照后响应的水力传导率值，并按活力分组。在第二个系列中，共进行了6次辐照实验和6次同步非辐照对照实验。使用的能量密度值为0.3571 J/cm（2）（n = 3）和0.4286 J/cm（2）（n = 3）。每隔2.5分钟分析一次辐照后的水力传导率值，按辐照对照组与未辐照对照组分组。结果如下：在第一个系列中，分析显示，从辐照后10分钟开始，活单层显示出水力传导率的统计学显著增加（p <0.001）。非活性单层细胞的水力传导率在统计学上显著降低。在第二个系列中，从照射后10分钟开始，照射组显示出与未照射对照组的显著差异（p <0.001）。结论：与基线值或同时未辐照对照相比，低通量二极管激光辐照增加了存活灌注TM细胞单层的水力传导率，同时降低了非存活单层的水力传导率。
Objective: To determine the effect of low- fluence diode laser irradiation upon the fluid perfusion characteristics of cultured human trabecular meshwork cell monolayers when placed in a specially designed testing apparatus and subjected to fluid flow driven by a hydrostatic pressure gradient. Methods: Two experimental series were conducted. In the first series, six low- fluence diode laser irradiation experiments were conducted using cultured human trabecular meshwork cell monolayers grown on filter supports. Upon reaching a steady state perfusion condition at approximately 5.0 mmHg, monolayers were irradiated at fluencies ranging from 0.2619 to 0.8571 J/cm(2) using a diode laser (lambda = 810 nm). Perfusion and data collection continued for 45 minutes post- irradiation, after which the monolayers were tested to determine post- experimental viability. Hydraulic conductivity values were analyzed for post- irradiation response in 2.5- minute intervals, grouped by viability. In the second series, a total of six irradiated experiments and six simulataneous nonirradiated control experiments were conducted. Fluence values of 0.3571 J/cm(2) ( n = 3) and 0.4286 J/cm(2) ( n = 3) were used. Hydraulic conductivity values were analyzed for post- irradiation response in 2.5- minute intervals, grouped by irradiated vs. nonirradiated control groups. Results: In the first series, analysis showed that the viable monolayers exhibited a statistically significant increase in hydraulic conductivity ( p < 0.001) from 10 minutes post- irradiation onward. The non- viable monolayers exhibited a statistically significant decrease in hydraulic conductivity. In the second series, irradiated groups showed a significant difference ( p < 0.001) from nonirradiated controls from 10 minutes post- irradiation onward. Conclusion: Low- fluence diode laser irradiation increases hydraulic conductivity in viable perfused TM cell monolayers when compared to baseline values or simultaneous nonirradiated controls while decreasing hydraulic conductivity in nonviable monolayers.