Sam35 of the mitochondrial protein sorting and assembly machinery is a peripheral outer membrane protein essential for cell viability
Sam35 of the mitochondrial protein sorting and assembly machinery is a peripheral outer membrane protein essential for cell viability
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DOI：
10.1074/jbc.c400120200
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发表时间：
2004-05-21
影响因子：
4.8
通讯作者：
Meisinger, C
中科院分区：
文献类型：
--
作者：
Milenkovic, D;Kozjak, V;Meisinger, C
线粒体外膜含有两种对细胞活力至关重要的整合蛋白，即外膜转位酶的Tom 40（TOM复合物）和分选和组装机器的Sam 50（SAM复合物）。在这里，我们报告的Sam 35，第一个外周线粒体外膜蛋白，是必不可少的细胞活力的鉴定。Sam 35（由酿酒酵母ORF YHR 083 w编码）是SAM复合物的新亚基，并且对于外膜β-桶蛋白（例如Tom 40和孔蛋白的前体）的组装途径至关重要。Sam 35对于内膜或基质靶向蛋白的输入是不需要的。SAM复合物中存在两种必需蛋白Sam 35和Sam 50，表明它在线粒体生物发生中起着核心作用。
The mitochondrial outer membrane contains two integral proteins essential for cell viability, Tom40 of the translocase of the outer membrane ( TOM complex) and Sam50 of the sorting and assembly machinery ( SAM complex). Here we report the identification of Sam35, the first peripheral mitochondrial outer membrane protein that is essential for cell viability. Sam35 ( encoded by the Saccharomyces cerevisiae ORF YHR083w) is a novel subunit of the SAM complex and is crucial for the assembly pathway of outer membrane beta- barrel proteins, such as the precursors of Tom40 and porin. Sam35 is not required for the import of inner membrane or matrix targeted proteins. The presence of two essential proteins in the SAM complex, Sam35 and Sam50, indicates that it plays a central role in mitochondrial biogenesis.