Microbial Metabolism in Ice and Brine at -5°C
Microbial Metabolism in Ice and Brine at -5°C
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DOI：
10.1111/j.1462-2920.2011.02485.x
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发表时间：
2011-08-01
影响因子：
5.1
通讯作者：
Skidmore, Mark L.
中科院分区：
文献类型：
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作者：
Bakermans, Corien;Skidmore, Mark L.
在 -5 摄氏度到 -32 摄氏度的温度下，在冰中观察到了代谢活动，但没有观察到生长。为了加深对冰中代谢的了解，我们同时检查了冰川分离菌 Sporosarcina sp. 的代谢的各个方面（C-14-乙酸盐利用、大分子合成和通过 CTC 还原的活力）。 B5 和金黄杆菌属 sp. V3519-10 在营养丰富的冰和盐水中在 -5 摄氏度下孵育 50 天。测得的醋酸盐利用率和大分子合成率在前 20 天内很高，表明冰和盐水中液体静脉网络的较低温度和较高盐浓度的调整。经过这种调整，两种生物体的生殖生长在盐水中都很明显，并且表明孢子八叠球菌属 (Sporosarcina sp.) 的繁殖生长。冰中的 B5 通过增加细胞数量和生物量来实现。金黄杆菌属在冰中孵育的 V3519-10 细胞仍保持活性。这些数据表明，低温和高盐浓度都不会抑制冰中的生长，但生活在冰中的某些其他方面会减慢生长速度，使其处于当前方法的检测限度内。这些结果表明，微生物在具有可比温度和足够营养的天然冰系统中生长是合理的，例如冰川和冰块中富含碎片的基底冰。
Metabolic activity, but not growth, has been observed in ice at temperatures from -5 degrees C to -32 degrees C. To improve understanding of metabolism in ice, we simultaneously examined various aspects of metabolism (C-14-acetate utilization, macromolecule syntheses and viability via reduction of CTC) of the glacial isolates Sporosarcina sp. B5 and Chryseobacterium sp. V3519-10 during incubation in nutrient-rich ice and brine at -5 degrees C for 50 days. Measured rates of acetate utilization and macromolecule syntheses were high in the first 20 days suggesting adjustment to the lower temperatures and higher salt concentrations of both the liquid vein network in the ice and the brine. Following this adjustment, reproductive growth of both organisms was evident in brine, and suggested for Sporosarcina sp. B5 in ice by increases in cell numbers and biomass. Chryseobacterium sp. V3519-10 cells incubated in ice remained active. These data indicate that neither low temperature nor high salt concentrations prohibit growth in ice, but some other aspect of living within ice slows growth to within the detection limits of current methodologies. These results imply that microbial growth is plausible in natural ice systems with comparable temperatures and sufficient nutrients, such as debris-rich basal ices of glaciers and ice masses.