Optimization of perfluoro nano-scale emulsions: The importance of particle size for enhanced oxygen transfer in biomedical applications
Optimization of perfluoro nano-scale emulsions: The importance of particle size for enhanced oxygen transfer in biomedical applications
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DOI：
10.1016/j.colsurfb.2012.04.011
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发表时间：
2012-10-01
影响因子：
5.8
通讯作者：
Stabler, Cherie L.
中科院分区：
文献类型：
--
作者：
Fraker, Christopher A.;Mendez, Armando J.;Stabler, Cherie L.
纳米级乳化长期以来一直被食品和化妆品行业利用，通过增加表面积与体积之比来最大限度地提高材料输送。最近，这些方法已被用于生物医学研究领域，以增强和控制所需试剂的输送，例如用于氧气输送的全氟化碳乳液。在这项工作中，我们评估了优化用于细胞应用的 PFC 乳液的关键因素。细胞毒性筛选显示，除用于乳液制造的一种全氟化碳、全氟辛基溴 (PFOB) 以及所使用的基于 PEG 的表面活性剂的特定 w% 限制外，各组分的细胞毒性极小。我们通过评估以下方面来优化稳定纳米级乳液的制造：成分材料、乳化时间和压力以及所得的粒径和时间稳定性。初始乳液尺寸很大程度上取决于所测试的乳液表面活性剂，其中 Pluronics 提供最小尺寸。纳米级乳液的时间稳定性与所使用的全氟化碳（包括全氟三丁胺 FC-43）直接相关。提供高度稳定的乳液，同时全氟萘烷。 PFD，随着时间的推移而合并。还对所得乳液的氧传质或扩散渗透性进行了表征。我们的研究发现颗粒尺寸是影响氧气传质的关键因素，因为胶束尺寸的增加会导致氧气扩散减少。总体而言，这项工作证明了准确表征乳化参数的重要性，以生成具有所需生物递送特性的稳定、可重复的乳液。 (C) 2012 Elsevier B.V. 保留所有权利。
Nano-scale emulsification has long been utilized by the food and cosmetics industry to maximize material delivery through increased surface area to volume ratios. More recently, these methods have been employed in the area of biomedical research to enhance and control the delivery of desired agents, as in perfluorocarbon emulsions for oxygen delivery. In this work, we evaluate critical factors for the optimization of PFC emulsions for use in cell-based applications. Cytotoxicity screening revealed minimal cytotoxicity of components, with the exception of one perfluorocarbon utilized for emulsion manufacture, perfluorooctylbromide (PFOB), and specific w% limitations of PEG-based surfactants utilized. We optimized the manufacture of stable nano-scale emulsions via evaluation of: component materials, emulsification time and pressure, and resulting particle size and temporal stability. The initial emulsion size was greatly dependent upon the emulsion surfactant tested, with pluronics providing the smallest size. Temporal stability of the nano-scale emulsions was directly related to the perfluorocarbon utilized, with perfluorotributylamine, FC-43. providing a highly stable emulsion, while perfluorodecalin. PFD, coalesced over time. The oxygen mass transfer, or diffusive permeability, of the resulting emulsions was also characterized. Our studies found particle size to be the critical factor affecting oxygen mass transfer, as increased micelle size resulted in reduced oxygen diffusion. Overall, this work demonstrates the importance of accurate characterization of emulsification parameters in order to generate stable, reproducible emulsions with the desired bio-delivery properties. (C) 2012 Elsevier B.V. All rights reserved.