Suppression of murine IgE responses with amino acid polymer/allergen conjugates. I. Preparation of poly-(N-methylglycine)/grass pollen extract conjugates using 4-(methylmercapto)phenyl-succinimidyl succinate as coupling reagent.
Suppression of murine IgE responses with amino acid polymer/allergen conjugates. I. Preparation of poly-(N-methylglycine)/grass pollen extract conjugates using 4-(methylmercapto)phenyl-succinimidyl succinate as coupling reagent.
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用氨基酸聚合物/过敏原缀合物抑制小鼠 IgE 反应。
DOI：
10.1159/000233721
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发表时间：
1985
期刊：
影响因子：
--
通讯作者：
G. P. Cottam
中科院分区：
文献类型：
--
作者：
N. Whittall;D. Moran;A. Wheeler;G. P. Cottam
开发了一种利用 4-(甲硫基)苯基酯基团的潜在活化电位的程序，用于受控、可重复地制备大分子缀合物。该酯作为琥珀桥桥基团的一部分，用于通过其 N 端仲胺将水溶性氨基酸聚合物聚（N-甲基甘氨酸）（聚肌氨酸）与草花粉混合物水提取物的亲核成分偶联。该产品对小鼠体内 IgE 合成具有可重复的、抗原特异性的抑制作用。
A procedure utilising the latent activating potential of the 4-(methylmercapto)phenyl ester group has been developed for the controlled, reproducible preparation of macromolecular conjugates. This ester, as part of a succinyl-bridging group, was used to couple the water-soluble, amino acid polymer, poly-(N-methylglycine) (polysarcosine), via its N-terminal secondary amine, with the nucleophilic components of the aqueous extract of a mixture of grass pollens. The products exhibit a reproducible, antigen-specific suppressive effect on the in vivo synthesis of IgE in mice.