CLONING, SEQUENCING AND EXPRESSION OF THE LIPASE GENE FROM PSEUDOMONAS-FRAGI IFO-12049 IN ESCHERICHIA-COLI
CLONING, SEQUENCING AND EXPRESSION OF THE LIPASE GENE FROM PSEUDOMONAS-FRAGI IFO-12049 IN ESCHERICHIA-COLI
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DOI：
10.1016/0014-5793(88)80980-3
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发表时间：
1988-12-19
期刊：
影响因子：
3.5
通讯作者：
INOUYE, S
中科院分区：
文献类型：
--
作者：
AOYAMA, S;YOSHIDA, N;INOUYE, S
使用表达文库分离来自脆弱假单胞菌IFO-12049的脂肪酶基因，并确定从核苷酸序列推导的脂肪酶的一级结构。它由277个氨基酸残基组成，蛋白为Mr29 966，与大肠杆菌表达的脂肪酶值接近。大肠杆菌。
The lipase gene fromPseudomonas fragiIFO‐12049 was isolated using the expression library and the primary structure of lipase deduced from the nucleotide sequence was determined. It is composed of 277 amino acid residues and a protein ofMr29 966, which was close to the value of the lipase expressed inE. coli.