Coenzyme Q10 supplementation down-regulates the increase of toll-like receptor 4 expressing monocytes in responses to 6-day intensive training in kendo athletes.
Coenzyme Q10 supplementation down-regulates the increase of toll-like receptor 4 expressing monocytes in responses to 6-day intensive training in kendo athletes.
复制标题
补充辅酶 Q10 可以下调剑道运动员 6 天强化训练后表达 Toll 样受体 4 的单核细胞的增加。
DOI：
10.1139/apnm-2014-0556
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发表时间：
2015
期刊：
影响因子：
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通讯作者：
Kono I.
中科院分区：
文献类型：
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作者：
1.Shimizu K;Kon M;Tanimura Y;Hanaoka Y;Akama T;Kono I.
本研究检测了Toll样受体4（TLR-4）表达的单核细胞和淋巴细胞亚群在运动员持续大强度运动训练中的变化，以及补充辅酶Q10（CoQ 10）对这些变化的影响。采用双盲方法，将18名日本男性剑道精英运动员随机分配到辅酶Q10补充组（n= 9）或安慰剂补充组（n= 9）。CoQ 10组的受试者每天服用300 mg CoQ 10，持续20天。安慰剂组受试者服用相同剂量的安慰剂。所有受试者在研究期间每天练习剑道5.5小时，连续6天。在训练前2周、训练第1天（第1天）、第3天（第3天）和第5天（第5天）以及训练期结束后1周采集血样（培训后）以确定TLR-4+/CD 14+单核细胞和淋巴细胞亚群（CD 3+、CD 4+、CD 8+、CD 28 +/CD 4+、CD 28 +/CD 8+和CD 56 +/CD 3 −细胞）。TLR-4+/CD 14+细胞的组×时间相互作用未达到显著性（p= 0.08）。在辅酶Q10组中，TLR-4+/CD 14+细胞的绝对数量仅在第5天显著较高。安慰剂组在第3天、第5天和训练后显示TLR-4+/CD 14+细胞的绝对数量显著增加（p< 0.05）。任何淋巴细胞亚群均无显著的组×时间相互作用。在第3天，两组的CD 3+、CD 8+和CD 56 +/CD 3 −细胞均显著降低（p< 0.05）。总之，辅酶Q10补充剂可能会下调TLR-4表达单核细胞的增加，以应对连续剧烈运动训练的剑道运动员。
This study examined changes in toll-like receptor 4 (TLR-4)-expressing monocytes and lymphocyte subpopulations in response to continuous intensive exercise training in athletes, as well as the effect of coenzyme Q10 (CoQ10) supplementation on these changes. Eighteen male elite kendo athletes in Japan were randomly assigned to a CoQ10-supplementation group (n= 9) or a placebo-supplementation group (n= 9) using a double-blind method. Subjects in the CoQ10 group took 300 mg CoQ10 per day for 20 days. Subjects in the placebo group took the same dosage of placebo. All subjects practiced kendo 5.5 h per day for 6 consecutive days during the study period. Blood samples were collected 2 weeks before training, on the first day (day 1), third day (day 3), and fifth day of training (day 5), and 1 week after the training period (post-training) to ascertain TLR-4+/CD14+monocyte and lymphocyte subpopulations (CD3+, CD4+, CD8+, CD28+/CD4+, CD28+/CD8+, and CD56+/CD3−cells) using flow cytometry analysis. The group × time interaction for TLR-4+/CD14+cells did not reach significance (p= 0.08). Within the CoQ10 group, the absolute number of TLR-4+/CD14+cells was significantly higher only at day 5. The placebo group showed a significant increase in the absolute number of TLR-4+/CD14+cells at day 3, day 5, and post-training (p< 0.05). There was no significant group × time interaction for any lymphocyte subpopulation. CD3+, CD8+, and CD56+/CD3−cells were significantly reduced at day 3 in both groups (p< 0.05). In conclusion, CoQ10 supplementation might downregulate the increase of TLR-4-expressing monocytes in response to continuous strenuous exercise training in kendo athletes.