Use of a digital brain atlas to compare the distribution of NGF- and bFGF-protected cholinergic neurons.
Use of a digital brain atlas to compare the distribution of NGF- and bFGF-protected cholinergic neurons.
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使用数字脑图谱来比较 NGF 和 bFGF 保护的胆碱能神经元的分布。
DOI：
10.1002/cne.903090104
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发表时间：
1991
期刊：
影响因子：
--
通讯作者：
Hefti,F
中科院分区：
文献类型：
--
作者：
Schwaber,JS;Due,BR;Rogers,WT;Junard,EO;Sharma,A;Hefti,F
通过使用计算机数据采集系统和数字脑图谱（可获得定量和分布信息），比较碱性成纤维细胞生长因子和神经生长因子在预防损伤诱导的隔胆碱能神经元消失方面的有效性。成年大鼠给予单侧海马伞部分横切，然后每天脑室内注射一种生长因子，持续15天。鉴于神经生长因子受体与胆碱乙酰转移酶在这些区域的高度共定位，通过神经生长因子受体免疫反应性鉴定胆碱能神经元。在三维脑图谱的背景下绘制其位置，以分析对照脑和每种生长因子处理动物脑中胆碱能神经元的相对分布。部分切断海马伞引起的胆碱能细胞消失仅限于内侧隔核和Broca斜角带的垂直支。在受影响的区域内，胆碱能细胞消失的严重程度从隔核的前部到后部逐渐增加。两种生长因子均能阻止隔内侧核和斜角带垂直支胆碱能胞体的消失。在损伤对照组动物中，单侧细胞消失占未损伤侧胆碱能神经元数量的53.5%。神经生长因子和碱性成纤维细胞生长因子分别将这种消失减少到13%和28%。胆碱能细胞的分布是相同的动物与每种生长因子，这表明两种生长因子保护相同的胆碱能神经元的人口。
The effectiveness of basic fibroblast growth factor and nerve growth factor in preventing the lesion‐induced disappearance of septal cholinergic neurons was compared by using a computerized data‐acquisition system and a digital brain atlas that yielded quantitative and distributional information. Adult rats were given unilateral partial transections of the fimbria and then received daily intraventricular injection of one of the growth factors for 15 days. Given the high degree of co‐localization of nerve growth factor receptors with choline acetyltransferase in these areas, cholinergic neurons were identified by nerve growth factor receptor immunoreactivity. Their locations were plotted in the context of a three‐dimensional brain atlas permitting the analysis of relative distributions of cholinergic neurons in control brains and those of animals treated with each growth factor. The cholinergic cell disappearance induced by the partial fimbrial transection was restricted to the medial septal nucleus and the vertical limb of the diagonal band of Broca. Within the affected areas cholinergic cell disappearance increased gradually in severity from anterior to posterior levels of the septal nucleus. Both growth factors prevented the disappearance of cholinergic cell bodies in medial septal nucleus and vertical limb of the diagonal band. In lesioned control animals the unilateral cell disappearance amounted to 53.5% of the number of cholinergic neurons of the unlesioned side. Nerve growth factor and basic fibroblast growth factor reduced this disappearance to 13% and 28%, respectively. The distribution of cholinergic cells was the same in animals treated with each growth factor, suggesting that the two growth factors protect the same population of cholinergic neurons.