Structural Proteins of Rabies Virus
Structural Proteins of Rabies Virus
复制标题
狂犬病病毒的结构蛋白
作者：
F. Sokol;D. Stanček;H. Koprowski
纯化的狂犬病病毒粒子，未标记或标记的放射性氨基酸或D-氨基葡萄糖，解离成他们的多肽与十二烷基硫酸钠在还原环境中的处理和分馏的十二烷基硫酸钠的聚丙烯酰胺凝胶电泳。通过将单个多肽的迁移速率与已知分子量的蛋白质标记物的迁移速率进行比较来估计单个多肽的分子量。纯化的病毒核衣壳和包膜成分的混合物，分离自脱氧胆酸钠破坏的病毒粒子，通过相同的程序进行分析。根据分离组分的比例、病毒核糖核酸的已知分子量和核衣壳的化学组成估计每种多肽的每个病毒粒子的分子数。核衣壳颗粒的蛋白质部分估计由1，713个主要多肽分子（分子量，62，000道尔顿）和76个次要多肽分子（分子量，55，000道尔顿）组成。除了1，783个糖蛋白组分分子（分子量，80，000道尔顿）外，病毒包膜分别含有789和1，661个其他两种多肽分子（分子量，40，000和25，000道尔顿）。
Purified rabies virions, unlabeled or labeled with radioactive amino acids or d-glucosamine, were dissociated into their polypeptides by treatment with sodium dodecyl sulfate in a reducing environment and fractionated by electroiphoresis in sodium dodecyl sulfate-containing polyacrylamide gel. The molecular weights of individual polypeptides were estimated by comparison of their rate of migration with that of protein markers of known molecular weight. Purified viral nucleocapsid and a mixture of envelope components, isolated from virions disrupted by sodium deoxycholate, were analyzed by the same procedure. The number of molecules per virion of each polypeptide was estimated from the proportions of the separated components, the known molecular weight of the viral ribonucleic acid, and the chemical composition of the nucleocapsid. The protein moiety of the nucleocapsid particle was estimated to consist of 1,713 molecules of a major polypeptide (molecular weight, 62,000 daltons) and 76 molecules of a minor polypeptide (molecular weight, 55,000 daltons). In addition to 1,783 molecules of a glycoprotein component (molecular weight, 80,000 daltons), the viral envelope contains 789 and 1,661 molecules, respectively, of two other polypeptides (molecular weight, 40,000 and 25,000 daltons).