Upregulation of Renin-angiotensin, Endothelin and C-type Natriuretic Peptide in Rat Glomerulus with Bilateral Ureteral Obstruction
Upregulation of Renin-angiotensin, Endothelin and C-type Natriuretic Peptide in Rat Glomerulus with Bilateral Ureteral Obstruction
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发表时间：
2006
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通讯作者：
E. Bae;S. Kim
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作者：
E. Bae;S. Kim
本研究旨在探讨肾素-血管紧张素-醛固酮系统（RAAS）、内皮素（ET）和局部利钠肽（NP）系统对实验性双侧输尿管梗阻大鼠肾小球病的影响。 Sprague-Dawley雄性大鼠（体重）通过近端输尿管结扎双侧阻塞24小时。以相同的方式处理对照大鼠，不同之处在于不进行结扎。采用分级筛法从皮质分离肾小球，采用实时聚合酶链式反应测定局部肾素-血管紧张素系统（RAS）、醛固酮合酶（CYP11B2）、内皮素-1（ET-1）和NP系统的mRNA表达量。双侧输尿管梗阻后，肾素、血管紧张素转换酶1、ET-1 mRNA表达均升高，而血管紧张素转换酶2的mRNA表达无变化。 CYP11B2和血管紧张素II受体的表达没有改变。 C型利钠肽（CNP）表达增加，但其受体（利钠肽受体-B）没有变化。我们认为局部RAS和ET的上调在进行性肾小球损伤中发挥作用，而CNP活性的增强也在肾小球梗阻性尿路病变中发挥代偿作用。
The present study was designed to investigate the effects renin-angiotensin-aldosterone system (RAAS), endothelin (ET) and local natriuretic peptide (NP) system for glomerulopathy induced in the experimental bilateral ureteral obstructive rats. Sprague-Dawley male rats ( body weight) were bilaterally obstructed by ligation of the proximal ureters for 24 hours. Control rats were treated in the same ways, except that no ligature was made. The glomeruli were isolated from cortex by graded sieve methods, and the mRNA expressions of local renin-angiotensin system (RAS), aldosterone synthase (CYP11B2), endothelin-1 (ET-1) and NP system were determined by real-time polymerase chain reaction. Following the bilateral ureteral obstruction, the mRNA expressions of renin, angiotensin converting enzyme 1 as well as ET-1 were increased, while that of angiotensin converting enzyme 2 was not changed. The expressions of CYP11B2 and angiotensin II receptors were not changed. C-type natriuretic peptide (CNP) expression was increased, while its receptors (natriuretic peptide receptor-B) were not changed. We suggest that the upregulation of local RAS and ET playa role in the progressive glomerular injury, and that the enhanced CNP activity also plays a compensatory role in obstructive uropathy in the glomerulus.