Transcriptome response to pollutants and insecticides in the dengue vector Aedes aegypti using next-generation sequencing technology
Transcriptome response to pollutants and insecticides in the dengue vector Aedes aegypti using next-generation sequencing technology
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DOI：
10.1186/1471-2164-11-216
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发表时间：
2010-03-31
期刊：
影响因子：
4.4
通讯作者：
Reynaud, Stephane
中科院分区：
文献类型：
--
作者：
David, Jean-Philippe;Coissac, Eric;Reynaud, Stephane
背景：控制传播传染病的蚊子主要依靠使用化学杀虫剂。然而，蚊子控制计划现在受到杀虫剂抗药性的出现的威胁。到目前为止，大多数研究工作都集中在阐明遗传抗性的分子基础上。人们较少注意蚊子对杀虫剂和污染物的短期反应，因为这可能对杀虫剂的药效产生重大影响。利用LongSAGE和Solexa测序相结合的方法对登革热媒介埃及伊蚊幼虫在亚致死剂量的3种化学杀虫剂和3种人为污染下暴露48h后进行了深度转录组分析。结果：共测序了3000万个20bp的cDNA标签，定位于蚊子基因组并进行了聚类，代表了6850个已知基因和4868个不在预测基因范围内的额外簇。暴露在杀虫剂或人为污染物中的蚊子表现出相当大的转录组改变。编码角质层蛋白、转运蛋白和参与线粒体呼吸链和解毒过程的酶的基因尤其受到影响。结论：本研究方法是研究基因组测序生物精细转录组变异的有效方法，可为新转录本的检测提供有用的信息。在生物层面上，尽管浓度很低，没有明显的表型效应，但这些外来物质对蚊子转录的重大影响提出了重要的问题，即人为污染物对生态系统的“隐性影响”以及对病媒控制的后果。
Background: The control of mosquitoes transmitting infectious diseases relies mainly on the use of chemical insecticides. However, mosquito control programs are now threatened by the emergence of insecticide resistance. Hitherto, most research efforts have been focused on elucidating the molecular basis of inherited resistance. Less attention has been paid to the short-term response of mosquitoes to insecticides and pollutants which could have a significant impact on insecticide efficacy. Here, a combination of LongSAGE and Solexa sequencing was used to perform a deep transcriptome analysis of larvae of the dengue vector Aedes aegypti exposed for 48 h to sub-lethal doses of three chemical insecticides and three anthropogenic pollutants.Results: Thirty millions 20 bp cDNA tags were sequenced, mapped to the mosquito genome and clustered, representing 6850 known genes and 4868 additional clusters not located within predicted genes. Mosquitoes exposed to insecticides or anthropogenic pollutants showed considerable modifications of their transcriptome. Genes encoding cuticular proteins, transporters, and enzymes involved in the mitochondrial respiratory chain and detoxification processes were particularly affected. Genes and molecular mechanisms potentially involved in xenobiotic response and insecticide tolerance were identified.Conclusions: The method used in the present study appears as a powerful approach for investigating fine transcriptome variations in genome-sequenced organisms and can provide useful informations for the detection of novel transcripts. At the biological level, despite low concentrations and no apparent phenotypic effects, the significant impact of these xenobiotics on mosquito transcriptomes raise important questions about the 'hidden impact' of anthropogenic pollutants on ecosystems and consequences on vector control.