Seasonal changes in picocyanobacterial diversity as revealed by pyrosequencing in temperate waters of the East China Sea and the East Sea
Seasonal changes in picocyanobacterial diversity as revealed by pyrosequencing in temperate waters of the East China Sea and the East Sea
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DOI：
10.3354/ame01669
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发表时间：
2013-01-01
影响因子：
1.4
通讯作者：
Shim, JaeSeol
中科院分区：
文献类型：
--
作者：
Choi, Dong Han;Noh, Jae Hoon;Shim, JaeSeol
为了解温带海域微囊藻多样性的季节和空间变化，对中国东海和东海3个站位的微囊藻丰度、遗传多样性和环境变量进行了调查。利用16S-23S内转录间隔区序列的条码扩增产物焦磷酸测序技术，对36个样品进行了苦味蓝细菌多样性的研究。通过焦磷酸测序，获得了27个不同的聚球藻和原氯球藻类的序列，显示出边缘水域具有较高的异氰藻多样性和明显的季节变化。冬、春季以沿海和冷水适应聚球藻I和IV占优势，夏季和秋季以暖水适应聚球藻II和原氯球HLII生态型占优势。此外，聚球藻分支III、V、VII和5.3-I以及原氯球菌LLI在夏季和初冬机会性地占据了不同的生态位。在这些温带边缘海中，微囊藻多样性的季节分布主要受温度和营养水平的控制。此外，邻近水团的季节环流模式是决定水的物理化学性质和微囊藻多样性的关键因素。
To elucidate the seasonal and spatial changes in picocyanobacterial diversity in temperate waters, the abundance and genetic diversity of picocyanobacteria and environmental variables were investigated at 3 stations located in the East China Sea and the East Sea. Barcoded amplicon pyrosequencing of 16S-23S internal transcribed spacer sequences was applied to study picocyanobacterial diversity using 36 samples. Through pyrosequencing, sequences belonging to 27 distinct Synechococcus and Prochlorococcus clades were retrieved, which showed high picocyanobacterial diversity and obvious seasonal variation in marginal waters. The coastal and cold water-adapted Synechococcus Clades I and IV were dominant during winter and spring, whereas the warm water-adapted Synechococcus Clade II and Prochlorococcus HLII ecotype were dominant during the summer and autumn. Further, Synechococcus Clades III, V, VII and 5.3-I as well as Prochlorococcus LLI opportunistically occupied distinct niches in the summer and early winter. In these temperate marginal seas, the seasonal distribution of picocyanobacterial diversity was mainly controlled by temperature and nutrient level. In addition, the seasonal circulation pattern of adjacent water masses was a key determinant of the physicochemical properties of water and consequently of picocyanobacterial diversity.