Complete nucleotide sequence of the neuraminidase gene of influenza B virus.
Complete nucleotide sequence of the neuraminidase gene of influenza B virus.
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乙型流感病毒神经氨酸酶基因的完整核苷酸序列。
DOI：
10.1073/pnas.79.22.6817
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发表时间：
1982
影响因子：
11.1
通讯作者：
Choppin,PW
中科院分区：
文献类型：
--
作者：
Shaw,MW;Lamb,RA;Erickson,BW;Briedis,DJ;Choppin,PW
利用克隆的病毒粒子RNA第6片段cDNA拷贝及其相应mRNA，获得了流感病毒B/Lee/40神经氨酸酶基因的完整核苷酸序列。RNA片段包含1557个病毒特异性核苷酸，最长的开放阅读框编码的蛋白质共有466个氨基酸，分子量为51721。与甲型流感病毒神经氨酸酶的情况一样，推断出的乙型流感蛋白的氨基酸序列包括靠近氨基末端的单个疏水区域，该区域将能够跨越病毒或细胞膜的脂质双分子层。蛋白质中有四个潜在的糖基化位点，其中两个位于氨基端疏水区附近。与甲型流感病毒神经氨酸酶的核苷酸和氨基酸序列比较发现，在分子的中心部分有7个广泛的同源区域，包括12个保守的半胱氨酸残基。末端部分的其他5个半胱氨酸残基也被保守。
The complete nucleotide sequence of the neuraminidase gene of influenza virus B/Lee/40 was derived from a cloned cDNA copy of virion RNA segment 6 and its corresponding mRNA. The RNA segment contains 1,557 virus-specific nucleotides, and the protein encoded by the longest open reading frame has a total of 466 amino acids with a molecular weight of 51,721. As is the case with the influenza A virus neuraminidases, the deduced amino acid sequence of the influenza B protein includes a single hydrophobic region near the amino terminus which would be capable of spanning the lipid bilayer of the viral or cell membrane. There are four potential glycosylation sites in the protein, two of which are near the amino-terminal hydrophobic region. Comparisons of the nucleotide and amino acid sequences with those of influenza A virus neuraminidases revealed seven regions of extensive homology within the central portion of the molecules, including 12 conserved cysteine residues. Five other cysteine residues in the terminal portions were also conserved.