Purification and characterization of RNase P from Clostridium sporogenes.
Purification and characterization of RNase P from Clostridium sporogenes.
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产孢梭菌 RNase P 的纯化和表征。
DOI：
10.1111/j.1365-2958.1990.tb00718.x
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发表时间：
1990
影响因子：
3.6
通讯作者：
Marsh,TL
中科院分区：
文献类型：
--
作者：
Roselli,DM;Marsh,TL
RNase P是一种多亚基酶，负责精确加工所有tRNA的5′末端。产孢梭菌的RNA亚基已部分纯化和鉴定。该RNA约400个核苷酸长，在成熟的tRNA 5′末端进行精确的核酸内切切割。RNA需要中等浓度的Mg 2+（20 mM）和相对高浓度的NH4Cl（800 mM）才能获得最佳活性。Mn2+在2mM时有效地取代Mg2+。Zn 2+、Ni 2+、Ca 2+和Co 2+对刺激活性无效。一般来说，离子半径越大，单价离子越有效（NH+4Cs>Rb>K>Na）。与枯草芽孢杆菌（Bacillussubtilis）相比，C.产孢核糖核酸酶P RNA在（NH_4）_2SO_4中的活性显著高于NH_4Cl。
RNase P is a multi‐subunit enzyme responsible for the accurate processing of the 5′ terminus of all tRNAs. The RNA subunit fromClostridium sporogeneshas been partially purified and characterized. The RNA is approximately 400 nucleotides long and makes a precise endonucleolytic cleavage at the mature 5′ terminus of tRNA. The RNA requires moderate concentrations of Mg2+(20mM) and relatively high concentrations of NH4Cl (800mM) for optimal activity. Mn2+effectively substitutes for Mg2+at 2mM. Zn2+, Ni2+, Ca2+, and Co2+are ineffective at stimulating activity. Monovalent ions are, in general, more effective the greater the ionic radius (NH+4Cs>Rb>K>Na). In contrast to the activity ofBacillus subtilis, C. sporogenesRNase P RNA is significant more active in (NH4)2SO4than in NH4Cl.