Induced Pluripotent Stem Cells (iPSCs) Derived from Different Cell Sources and their Potential for Regenerative and Personalized Medicine
Induced Pluripotent Stem Cells (iPSCs) Derived from Different Cell Sources and their Potential for Regenerative and Personalized Medicine
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DOI：
10.2174/1566524011313050010
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发表时间：
2013-06-01
影响因子：
2.5
通讯作者：
Itskovitz-Eldor, J.
中科院分区：
文献类型：
--
作者：
Shtrichman, R.;Germanguz, I.;Itskovitz-Eldor, J.
人诱导多能干细胞（hiPSCs）作为再生医学的强大祖细胞来源具有巨大的潜力。这项新技术还可以衍生出用于个性化医疗的患者特异性细胞，例如用于个人药物筛选和毒理学。然而，iPSCs的生物学特性尚未完全了解，其与人类胚胎干细胞（hESCs）的相似性仍未得到解决。多能干细胞之间的差异，由于其原始组织或细胞来源，以及用于其衍生的实验方案，显着影响表观遗传特性和分化潜力。在这里，我们回顾了iPSCs在再生和个性化医学方面的潜力，并评估了它们的表达模式、表观遗传记忆和与其亲本组织来源相关的分化能力。我们还总结了在生物学研究和药物发现中应用的患者特异性iPSCs；并审查为了将iPSC技术用于临床应用而必须克服的风险。
Human induced pluripotent stem cells (hiPSCs) have great potential as a robust source of progenitors for regenerative medicine. The novel technology also enables the derivation of patient-specific cells for applications to personalized medicine, such as for personal drug screening and toxicology. However, the biological characteristics of iPSCs are not yet fully understood and their similarity to human embryonic stem cells (hESCs) is still unresolved. Variations among iPSCs, resulting from their original tissue or cell source, and from the experimental protocols used for their derivation, significantly affect epigenetic properties and differentiation potential. Here we review the potential of iPSCs for regenerative and personalized medicine, and assess their expression pattern, epigenetic memory and differentiation capabilities in relation to their parental tissue source. We also summarize the patient-specific iPSCs that have been derived for applications in biological research and drug discovery; and review risks that must be overcome in order to use iPSC technology for clinical applications.