Interaction of ZnO Nanoparticles with Microbes-A Physio and Biochemical Assay
Interaction of ZnO Nanoparticles with Microbes-A Physio and Biochemical Assay
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DOI：
10.1166/jbn.2011.1343
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发表时间：
2011-12-01
影响因子：
2.9
通讯作者：
Vijayaraghavan, Rajagopalan
中科院分区：
文献类型：
--
作者：
Padmavathy, Nagarajan;Vijayaraghavan, Rajagopalan
研究了纳米氧化锌对多种微生物的抑菌活性，并探讨了纳米氧化锌对大肠杆菌的抑菌机理。通过分析细菌细胞的生长、渗透性和形态，实验结果表明，1 mmoL（81.4 μ g）纳米ZnO可以完全抑制10（7）cfu/mL的细菌细胞在液体Luria-Betrani培养基中的生长。在生化研究中，已经观察到，纳米ZnO导致蛋白质的泄漏。化学发光分析证明活性氧物种进入活性状态的产生。当E.大肠杆菌经纳米ZnO处理后，透射电镜和荧光显微镜观察到细菌细胞内有许多凹坑，细胞破碎。用原子吸收分光光度法测定了锌离子的释放量。说明纳米ZnO能够破坏细菌细胞膜的通透性。实验结果表明，纳米ZnO通过产生活性氧，破坏了细菌细胞膜结构，抑制了某些膜酶的活性，从而导致大肠杆菌的死亡。大肠杆菌最终死亡。
The antibacterial activity of ZnO nanoparticles (nano ZnO) on various microorganisms has been investigated in this study and acting mechanism is proposed for E. coli a model organism by analyzing the growth, permeability and morphology of the bacterial cells. The experimental results indicated 1 mmoL (81.4 mu g) nano ZnO could completely inhibit the growth of 10(7) cfu/mL bacterial cells in liquid Luria-Betrani medium. In the biochemical study, it has been observed that, nano ZnO resulted in the leakage of proteins. Chemiluminescence assay proved the generation of the reactive oxygen species into active state. When the cells of E. coli were exposed to nano ZnO, many pits were observed in bacterial cells by TEM and Fluorescence microscopy and the cells were found to be fragmentary. Released zinc ion from has been monitored by AAS. It suggested that nano ZnO is able to destroy the permeability of the bacterial membranes. Our study demonstrated that nano ZnO damages the structure of bacterial cell membrane and depressed the activity of some membranous enzymes by ROS production which caused E. coli bacteria to die eventually.