Trans Chromosomal Methylation in Arabidopsis hybrids
Trans Chromosomal Methylation in Arabidopsis hybrids
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DOI：
10.1073/pnas.1201043109
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发表时间：
2012-02-28
影响因子：
11.1
通讯作者：
Dennis, Elizabeth S.
中科院分区：
文献类型：
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作者：
Greaves, Ian K.;Groszmann, Michael;Dennis, Elizabeth S.
拟南芥C24和直立拟南芥的杂种后代已经改变了甲基组。最常见的变化发生在父母甲基化水平不同的基因座上。非加性甲基化模式中存在特定于上下文的偏向，相对于亲本，(M)CG普遍增加，(CHH)-C-m降低。这些变化是两种主要机制的结果，跨染色体甲基化和跨染色体去甲基化，其中一个亲本等位基因的甲基化水平改变为与另一个亲本的甲基化水平相似。甲基化改变的区域在基因区域周围丰富，并且通常与siRNA水平的变化相关。我们确定了表达改变的基因的例子，可能是由于甲基化改变，并表明在C24和LER材料之间的杂交中，表观遗传控制在产生改变的转录水平方面可能是重要的，这可能有助于杂交种生物量的增加。
The heterotic hybrid offspring of Arabidopsis accessions C24 and Landsberg erecta have altered methylomes. Changes occur most frequently at loci where parental methylation levels are different. There are context-specific biases in the nonadditive methylation patterns with (m)CG generally increased and (CHH)-C-m decreased relative to the parents. These changes are a result of two main mechanisms, Trans Chromosomal Methylation and Trans Chromosomal deMethylation, where the methylation level of one parental allele alters to resemble that of the other parent. Regions of altered methylation are enriched around genic regions and are often correlated with changes in siRNA levels. We identified examples of genes with altered expression likely to be due to methylation changes and suggest that in crosses between the C24 and Ler accessions, epigenetic controls can be important in the generation of altered transcription levels that may contribute to the increased biomass of the hybrids.