Effect of endothelin-1 on hepatic damage induced by endotoxin.
Effect of endothelin-1 on hepatic damage induced by endotoxin.
复制标题
内皮素1对内毒素所致肝损伤的影响。
DOI：
--
复制
发表时间：
2000
期刊：
影响因子：
--
通讯作者：
Ji
中科院分区：
文献类型：
--
作者：
Baohua Liu;N. Xiao;Hui;Ji
目的：观察内皮素-1 （ET-1）对内毒素所致肝损害的影响。方法：将90只大鼠随机分为对照组（C组）、内毒素处理组（LPS组）和内毒素+ ET-1抗体处理组（LEA组）。观察ET-1浓度的变化及ET-1 mRNA的转录和表达。分别在生理盐水、内毒素（10 mg/kg）和ET-1抗体（2 ml/kg）给药后3、6、9、12、24小时观察血清谷丙转氨酶（GPT）、肝乳酸脱氢酶（LDH）、三磷酸腺苷（ATP）和丙二醛（MDA）水平。结果：内毒素血症后大鼠血浆和肝脏ET-1浓度及肝脏ET-1 mRNA表达均显著升高。肝脏ET-1水平与ATP浓度呈负相关，与MDA浓度呈正相关。ET-1抗体能部分保护肝脏免受内毒素损伤。结论：这些结果提示内毒素可能在转录和翻译水平上导致ET-1合成增加。ET-1可能在内毒素血症中导致肝损伤。
OBJECTIVE: To observe effect of endothelin-1 (ET-1) on hepatic damage induced by endotoxin. METHODS: A total of 90 rats were randomly divided into control group (group C), endotoxin treated group (group LPS) and endotoxin plus ET-1 antibody treated group (group LEA). An observation was done on the changes of ET-1 concentration, and transcription and expression of ET-1 mRNA. Plasma glutamic pyruvic-transaminase enzyme (GPT), hepatic lactate dehydrogenase (LDH), adenosine triphosphate (ATP) and malondialdehyde (MDA) were also observed at 3, 6, 9, 12, 24 hours after saline, endotoxin (10 mg/kg) and ET-1 antibody (dalubine 1:2000, 2 ml/kg) administration. RESULTS: The results indicated that the concentration of plasma and hepatic ET-1 and expression of ET-1 mRNA in liver significantly increased following endotoxemia. The hepatic ET-1 levels were inversely correlated with the ATP concentration, and positively related to the MDA concentration. ET-1 antibody could partially protect the liver against damage induced by endotoxin. CONCLUSIONS: These results suggest that endotoxin may, on transcription and translation level, lead to an increase of ET-1 in synthesis. ET-1 may contribute to hepatic damage during endotoxemia.