A somatic role for eyes absent (eya) and sine oculis (so) in Drosophila spermatocyte development
A somatic role for eyes absent (eya) and sine oculis (so) in Drosophila spermatocyte development
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DOI：
10.1016/s0012-1606(03)00127-1
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发表时间：
2003-06-01
影响因子：
2.7
通讯作者：
DiNardo, S
中科院分区：
文献类型：
--
作者：
Fabrizio, JJ;Boyle, M;DiNardo, S
索马和生殖细胞之间的相互作用是整个动物界精子发生的保守特征。在这份报告中，我们发现转录因子eyes absent（eya）和sine oculis（so），以前被证明在眼睛发育过程中起主要作用[Cell 91（1997），881]，在果蝇精母细胞正常发育所需的睾丸体细胞囊肿细胞中。Eya突变体睾丸表现出退化的年轻精母细胞。嵌合体分析揭示了eya和so的体细胞需求，因为这两个基因在精母细胞发育的种系中都不是必需的。免疫定位分析支持这种体细胞的作用，因为这两种蛋白质都定位在囊细胞核内的精母细胞分化从扩增精原细胞。使用已知的囊肿细胞标记的抗体，我们证明了退化的精母细胞在EYA突变体睾丸囊肿被包囊，排除了EYA在囊肿细胞活力的作用。最后，我们发现了睾丸中eya和so之间的遗传相互作用，这表明，就像在眼睛中一样，eya和so可能形成一个转录复合体，负责激活参与囊细胞分化和精母细胞发育的靶基因。(C)2003 Elsevier Science（美国）。All rights reserved.
Interactions between the soma and the germline are a conserved feature of spermatogenesis throughout the animal kingdom. In this report, we find that the transcription factors eyes absent (eya) and sine oculis (so), previously shown to play major roles during eye development [Cell 91 (1997), 881] are each required in the somatic cyst cells of the testis for proper Drosophila spermatocyte development. eya mutant testes exhibit degenerating young spermatocytes. Mosaic analysis reveals a somatic requirement for both eya and so, in that neither gene is required in the germline for spermatocyte development. Immunolocalization analysis supports this somatic role, since both proteins are localized within cyst cell nuclei as spermatocytes differentiate from amplifying spermatogonia. Using antibodies against known cyst cell markers, we demonstrate that cysts of degenerating spermatocytes in eya mutant testes are encysted, ruling out a role for eya in cyst cell viability. Finally, we have uncovered a genetic interaction between eya and so in the testis, suggesting that, as in the eye, eya and so may form a transcription complex responsible for the activation of target genes involved in cyst cell differentiation and spermatocyte development. (C) 2003 Elsevier Science (USA). All rights reserved.