Novel Function of QM Protein of Shrimp (Penaeus Japonicus) in Regulation of Phenol Oxidase Activity by Interaction with Hemocyanin
Novel Function of QM Protein of Shrimp (Penaeus Japonicus) in Regulation of Phenol Oxidase Activity by Interaction with Hemocyanin
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DOI：
10.1159/000129640
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发表时间：
2008-04
影响因子：
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通讯作者：
Jianyang Xu;Suijie Wu;Xiaobo Zhang
中科院分区：
文献类型：
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作者：
Jianyang Xu;Suijie Wu;Xiaobo Zhang
自1991年被提出作为肿瘤抑制因子以来，QM蛋白在植物、动物和真菌中引起了广泛的研究。然而，迄今为止，QM蛋白的功能尚不清楚。本研究发现，抗病毒对虾QM基因（PjQM）显著上调，提示PjQM参与对虾免疫。GST下拉实验表明，PjQM蛋白与虾血青素和肌球蛋白相互作用，表明PjQM蛋白可能参与了虾免疫的prophenoloxidation （proPO）激活系统。通过RNAi分析，首次证实QM蛋白可以调节无脊椎动物免疫proPO激活系统中的关键酶苯酚氧化酶的活性。这一发现揭示了QM蛋白在节肢动物免疫应答中的一个新方面，有助于更好地理解节肢动物先天免疫的分子事件。
Since it was proposed to be a tumor suppressor in 1991, the QM protein has attracted intensive studies in plants, animals and fungi. Up to date, however, the function of QM protein remains unknown. In this investigation, it was found that the shrimp QM gene (designated as PjQM) was significantly up-regulated in virus- resistant shrimp, suggesting that the PjQM was involved in shrimp immunity. The GST pull-down assays showed that the PjQM protein interacted with shrimp hemocyanin and myosin, indicating that the PjQM protein might participate in prophenoloxidation (proPO) activation system of shrimp immunity. As revealed by RNAi assays, it was demonstrated for the first time that the QM protein could regulate the activity of phenol oxidase, a key enzyme in the proPO activation system of invertebrate immunity. This discovery showed a novel aspect of QM protein in arthropod immune response, which contributed a better understanding to the still poorly understood molecular events involved in innate immunity of arthropods.