CONTROL OF FRUCTOSE 2,6-BISPHOSPHATE LEVELS IN RAT MACROPHAGES BY GLUCOSE AND PHORBOL ESTER
CONTROL OF FRUCTOSE 2,6-BISPHOSPHATE LEVELS IN RAT MACROPHAGES BY GLUCOSE AND PHORBOL ESTER
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DOI：
10.1016/0014-5793(89)81444-9
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发表时间：
1989-07-17
期刊：
影响因子：
3.5
通讯作者：
SOBRINO, F
中科院分区：
文献类型：
--
作者：
BUSTOS, R;SOBRINO, F
本文观察了2，6-二磷酸果糖（Fru 2，6-P_2）在大鼠腹腔巨噬细胞中的存在。这些细胞具有活性的磷酸果糖激酶-2，其被柠檬酸盐减少，并且仅被甘油3-磷酸轻微抑制。通过加入1 μM Fru 2，6-P2，磷酸果糖激酶-1次最大活性增加2,6倍。不含葡萄糖的细胞孵育使Fru 2，6-P2的量降至零，但进一步加入5 mM葡萄糖使糖酯的水平增加20倍。此外，佛波酯的存在下加强了合成的Fru 2，6-P2。相反，苯异丙基腺苷或前列腺素F2α抑制Fru 2，6-P2的产生。
The presence of fructose 2,6-bisphosphate (Fru 2,6-P2) in elicited peritoneal macrophages of rat was examined. These cells possess an active phosphofructokinase-2 which is diminished by citrate and only slightly inhibited by glycerol 3-phosphate. Phosphofructokinase-1 submaximal activity was increased 26-fold by the addition of 1 μM Fru 2,6-P2. Incubation of cells without glucose decreased the amount of Fru 2,6-P2to zero, but further addition of 5 mM glucose increased the levels of the sugar ester 20-fold. In addition, the presence of phorbol ester potentiated the synthesis of Fru 2,6-P2. By contrast phenylisopropyladenosine or prostaglandin F2αinhibited the production of Fru 2,6-P2.