Analysis of the metabolic properties of maintenance hemodialysis patients with glucose-added dialysis based on high performance liquid chromatography quadrupole time-of-flight mass spectrometry.
Analysis of the metabolic properties of maintenance hemodialysis patients with glucose-added dialysis based on high performance liquid chromatography quadrupole time-of-flight mass spectrometry.
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DOI：
10.2147/tcrm.s49634
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发表时间：
2013
影响因子：
2.8
通讯作者：
Yin L
中科院分区：
文献类型：
--
作者：
Cui L;Meng Y;Xu D;Feng Y;Chen G;Hu B;Feng G;Yin L
本研究的目的是利用代谢组学比较使用含葡萄糖和无葡萄糖透析液治疗的维持性血液透析患者的代谢特性。采用高效液相色谱四极杆飞行时间质谱法对使用无葡萄糖透析液的 G (−) 组和使用添加葡萄糖的透析液（葡萄糖水平为 5.5 mmol/L）的 G (+) 组的透析前和透析后血清样品进行分析和测试。正交信号校正-偏最小二乘判别分析显示G(−)和G(+)组样本之间透析后代谢特性存在显着差异，并且G(+)组亮氨酸和二羟基前列腺素F2α的浓度高于G(−)组。然而，G (+) 组的反应性脂质动员和氨基酸释放标志物，如胆汁酸、天冬氨酸和缬氨酸，低于 G (-) 组。兴奋性神经递质天冬氨酸和磷酸化阿那达酰胺没有显着差异。液相色谱-串联质谱代谢组学研究表明，使用添加葡萄糖的透析液对维持性血液透析患者中枢神经系统的保护优于无葡萄糖透析液，但存在刺激氧化应激的潜在风险。
The purpose of this study was to compare the metabolic properties of maintenance hemodialysis patients treated with glucose-containing and glucose-free dialysate using metabonomics. Pre- and post-dialysis serum samples from group G (−) using glucose-free dialysate, and group G (+) using glucose-added dialysate (glucose levels were 5.5 mmol/L) were analyzed and tested with high performance liquid chromatography quadrupole time-of-flight mass spectrometry. Orthogonal signal correction–partial least squares discriminate analysis revealed a significant difference in the post-dialysis metabolic properties between samples from the G (−) and G (+) groups, and concentrations of leucine and dihydroxyprostaglandin F2α were higher in the G (+) group than in the G (−) group. However, markers of reactive lipid mobilization and amino acid release, such as bile acids, aspartate, and valine, were lower in the G (+) group than in the G (−) group. There were no significant differences in excitatory neurotransmitters aspartate and phosphorylated anandamide. Use of liquid chromatography-tandem mass spectrometry metabonomics indicated that using glucose-added dialysate was superior to glucose-free dialysate in the protection of the central nervous system of maintenance hemodialysis patients, but had potential risks in stimulating oxidative stress.