Changes in microRNA expression in the MG-63 osteosarcoma cell line compared with osteoblasts
Changes in microRNA expression in the MG-63 osteosarcoma cell line compared with osteoblasts
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DOI：
10.3892/ol.2012.866
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发表时间：
2012-11-01
期刊：
影响因子：
2.9
通讯作者：
Cai, Lin
中科院分区：
文献类型：
--
作者：
Hu, Hao;Zhang, Yi;Cai, Lin
骨肉瘤是最常见的原发恶性骨肿瘤，尤其是青少年和青壮年。早期诊断仍然是OS临床治疗中的一个重要问题，因为我们离全面了解OS的分子遗传学机制和所涉及的生物学还很远。此外，microRNAs(miRNAs或miRs)是一个小的非编码RNA的大家族，由于它们在体外和体内的基因表达调控中发挥着复杂的作用，因此可能会对OS有更好的理解。本研究采用miRNA微阵列和实时定量聚合酶链式反应(RT-qPCR)技术，研究了成骨细胞和OS细胞中miRNAs的差异表达。与成骨细胞系相比，共有268个miR-9、miR-99、miR-195、miR-148a和miR-181a高表达，miR-143、miR-145、miR-335和miR-539下调。这种差异表达可能有助于未来OS的诊断和预后预测，以及阐明OS的发生、发展和转移的潜在机制。对这些差异表达的miRNAs的生物信息学研究表明，它们能够以复杂而有效的方式调控OS的生物学行为。对其功能的进一步研究。其中的miRNAs可能为OS生物学和治疗提供新的见解。
Osteosarcoma (OS) is the most common primary malignant bone tumor, particularly in adolescents and young adults. Early diagnosis remains a significant problem in the clinical treatment of OS as we remain far from a comprehensive understanding of the molecular genetic mechanisms and the biology involved. In addition, microRNAs (miRNAs or miRs), a large family of small non-coding RNAs, may provide a greater understanding of OS as they play a complex role in gene expression regulation in vitro and in vivo. In the current study, the differential expression profiles of miRNAs between OS and osteoblast cell lines were investigated by miRNA microarrays and real-time quantitative PCR (RT-qPCR). A total of 268 miRNAs were identified that were significantly dysregulated in OS compared with the osteoblast cell line, including miR-9, miR-99, miR-195, miR-148a and miR-181a, which had been validated as overexpressed, and miR-143, miR-145, miR-335 and miR-539, which were confirmed to be downregulated. This differential expression may aid future OS diagnosis and prognosis prediction and illustration of the potential mechanisms in the oncogenesis, development and metastasis of OS. Bioinformatic research on these differentially expressed miRNAs suggests that they are able to regulate the biological behaviors of OS in a complex and effective manner. Further study on the function. of these miRNAs is likely to provide new insights into OS biology and treatment.