Experimental setup and first measurement of DNA damage induced along and around an antiproton beam
Experimental setup and first measurement of DNA damage induced along and around an antiproton beam
复制标题
实验设置和首次测量反质子束沿线和周围诱导的 DNA 损伤
DOI：
10.1140/epjd/e2010-00194-4
复制
发表时间：
2010
期刊：
影响因子：
--
通讯作者：
Kavanagh J
中科院分区：
文献类型：
--
作者：
Kavanagh J
放射治疗采用电离辐射来诱导癌细胞中的致命DNA损伤，同时最大限度地减少对健康组织的损伤。由于它们的能量沉积模式，理论上，与光子相比，离子可以实现更好的治疗效果。反质子被认为可以进一步增强DNA的损伤，本文的工作旨在建立和验证一种定量分析反质子对质粒和基因组DNA损伤的实验方法。免疫细胞化学被用来评估直接和间接暴露的人成纤维细胞在欧洲核子研究中心的126 MeV的反质子束的平台和布拉格峰区域照射的DNA损伤。照射后用抗γ-H2 AX抗体对细胞进行染色。γ-H2 AX焦点-剂量关系的定量与布拉格峰区域的线性增加一致。在布拉格峰和平台区检测到的焦点的定性分析表明显着的差异，突出了不同的严重程度的DNA损伤产生沿着particle path. Irradiation的脱盐质粒DNA与5戈伊的反质子在布拉格峰导致在所得溶液中的线性质粒的显着部分。
Radiotherapy employs ionizing radiation to induce lethal DNA lesions in cancer cells while minimizing damage to healthy tissues. Due to their pattern of energy deposition, better therapeutic outcomes can, in theory, be achieved with ions compared to photons. Antiprotons have been proposed to offer a further enhancement due to their annihilation at the end of the path. The work presented here aimed to establish and validate an experimental procedure for the quantification of plasmid and genomic DNA damage resulting from antiproton exposure. Immunocytochemistry was used to assess DNA damage in directly and indirectly exposed human fibroblasts irradiated in both plateau and Bragg peak regions of a 126 MeV antiproton beam at CERN. Cells were stained post irradiation with an anti-γ-H2AX antibody. Quantification of theγ-H2AX foci-dose relationship is consistent with a linear increase in the Bragg peak region. A qualitative analysis of the foci detected in the Bragg peak and plateau region indicates significant differences highlighting the different severity of DNA lesions produced along the particle path. Irradiation of desalted plasmid DNA with 5 Gy antiprotons at the Bragg peak resulted in a significant portion of linear plasmid in the resultant solution.
登录
查看更多内容
DOI：
10.1073/pnas.0830918100
发表时间：
2003-04-29
影响因子：
11.1
作者：
Rothkamm, K;Löbrich, M
通讯作者：
Löbrich, M
反质子湮灭的生物学有效性
DOI：
--
发表时间：
2004
期刊：
影响因子：
--
作者：
C. Maggiore;N. Agazaryan;N. Bassler;E. Blackmore;G. Beyer;J. DeMarco;M. Doser;C. Gruhn;M. Holzscheiter;T. Ichioka;K. Iwamoto;H. Knudsen;R. Landua;W. McBride;S. Møller;J. Petersen;J. Smathers;L. Skarsgard;T. Solberg;U. Uggerhøj;H. Withers;B. Wouters
通讯作者：
B. Wouters
水中反质子深度-剂量曲线
影响因子：
3.5
作者：
Niels Bassler;M. Holzscheiter;Oliver Jäkel;H. Knudsen;S. Kovačević
通讯作者：
S. Kovačević
影响因子：
5.7
作者：
M. Holzscheiter;N. Bassler;N. Agazaryan;G. Beyer;E. Blackmore;J. DeMarco;M. Doser;R. Durand;Oliver Hartley;K. Iwamoto;H. Knudsen;R. Landua;C. Maggiore;W. McBride;S. Møller;J. Petersen;L. Skarsgard;J. Smathers;T. Solberg;U. Uggerhøj;S. Vranjes;H. Withers;M. Wong;B. Wouters
通讯作者：
B. Wouters
反质子束可能的生物医学应用：聚焦辐射传输。
影响因子：
3.4
作者：
L. Gray;T. Kalogeropoulos
通讯作者：
T. Kalogeropoulos