Oxygen consumption in undifferentiated versus differentiated adipogenic mesenchymal precursor cells
Oxygen consumption in undifferentiated versus differentiated adipogenic mesenchymal precursor cells
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DOI：
10.1016/j.resp.2004.12.013
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发表时间：
2005-04-15
影响因子：
2.3
通讯作者：
Pallua, N
中科院分区：
文献类型：
--
作者：
von Heimburg, D;Hemmrich, K;Pallua, N
到目前为止，对于诸如大面积深度烧伤、肿瘤切除后或先天性缺陷等软组织缺损的矫正，还没有合适的植入材料。一种由有活力的脂肪前体细胞和优化基质组成的生物混合体可能有助于解决这一问题。从形态学上看，前脂肪细胞类似于成纤维细胞，并且尚未像分化的脂肪细胞那样形成大的细胞质脂滴。此外，前脂肪细胞比成熟脂肪细胞小，这使得移植后能更快地重新血管化。而且，移植的前脂肪细胞在体内能够形成脂肪组织，而成熟脂肪细胞的移植往往效果不佳，例如形成油囊肿或移植体萎缩。由于这些观察结果表明前脂肪细胞和脂肪细胞之间在代谢活性上存在差异，我们通过克拉克型氧电极测量呼吸作用，研究了受刺激进行分化的前脂肪细胞和成纤维细胞的耗氧量。前脂肪细胞的耗氧量明显低于成熟脂肪细胞。这种呼吸方面的优势以及未分化脂肪组织细胞更好的重新血管化能力，使得创新性移植得以发展，并表明前脂肪细胞是改善脂肪组织重建移植的有希望的工具。（c）2004爱思唯尔公司。版权所有。
To date, no adequate implant material for the correction of soft tissue defects such as after extensive deep burns, tumor resections or in congenital defects is available. A biohybrid composed of viable adipose precursor cells and an optimised matrix could help towards a solution. Morphologically, preadipocytes resemble fibroblasts and have not yet built a large cytoplasmic lipid droplet as found in differentiated adipocytes. Additionally, preadipocytes are smaller than mature adipocytes allowing a quicker revascularization after transplantation. Furthermore, transplanted preadipocytes can form adipose tissue in vivo whereas the transplantation of mature adipocytes often gives poor results, i.e. oil cysts or shrinkage of the transplant. Since these observations point to differences in metabolic activity between preadipocytes and adipocytes, we investigated the oxygen consumption of preadipocytes stimulated to undergo differentiation, and fibroblasts, by measuring the respiration with a Clark-type oxygen electrode. Preadipocytes had a significantly lower oxygen consumption than mature adipocytes. This advantage in respiration and the better revascularization of undifferentiated adipose tissue cells allow the development of innovative transplants and point to preadipocytes as promising tool to improve transplantations in adipose tissue reconstruction. (c) 2004 Elsevier B.V. All rights reserved.