Cranial expression of class 3 secreted semaphorins and their neuropilin receptors
Cranial expression of class 3 secreted semaphorins and their neuropilin receptors
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DOI：
10.1002/dvdy.10396
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发表时间：
2003-12-01
影响因子：
2.5
通讯作者：
Guthrie, S
中科院分区：
文献类型：
--
作者：
Chilton, JK;Guthrie, S
脑信号蛋白家族的化学排斥物及其受体神经纤毛蛋白参与多种细胞过程，包括轴突导向和细胞迁移。脑信号蛋白可以结合一个以上的神经纤毛蛋白或两者的异源二聚体，因此，详细了解它们的表达模式可能会揭示冗余或相互拮抗的可能情况。为了评估它们参与颅发育，我们克隆了Sema 3B和Sema 3F的鸡直系同源基因片段。然后，我们进行了mRNA原位杂交的所有6个3类semaphorins和神经纤毛在胚胎鸡头部。我们提出的证据，这些分子在脑干和发育中的头部，包括眼睛，耳朵，和鳃弓的时空调节。这些表达模式为脑信号蛋白和神经纤毛蛋白在轴突投射发育和颅结构形态发生中的功能分析提供了基础。(C)2003 Wiley-Liss，Inc.
The semaphorin family of chemorepellents and their receptors the neuropilins are implicated in a variety of cellular processes, including axon guidance and cell migration. Semaphorins may bind more than one neuropilin or a heterodimer of both, thus a detailed knowledge of their expression patterns may reveal possible cases of redundancy or mutual antagonism. To assess their involvement in cranial development, we cloned fragments of the chick orthologues of Sema3B and Sema3F. We then carried out mRNA in situ hybridisation of all six class 3 semaphorins and both neuropilins in the embryonic chick head. We present evidence for spatiotemporal regulation of these molecules in the brainstem and developing head, including the eye, ear, and branchial arches. These expression patterns provide a basis for functional analysis of semaphorins and neuropilins in the development of axon projections and the morphogenesis of cranial structures. (C) 2003 Wiley-Liss, Inc.