Rat corticotropin-releasing hormone gene: sequence and tissue-specific expression.
Rat corticotropin-releasing hormone gene: sequence and tissue-specific expression.
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DOI：
10.1210/mend-1-5-363
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发表时间：
1987-05
影响因子：
--
通讯作者：
R. Thompson;A. Seasholtz;E. Herbert
中科院分区：
文献类型：
--
作者：
R. Thompson;A. Seasholtz;E. Herbert
大鼠促肾上腺皮质激素释放激素（CRH）基因已被分离，并通过DNA序列分析进行了鉴定。该基因表现出与人类CRH基因相似的结构组织。编码整个大鼠CRH前体的核苷酸序列位于第二个外显子上，而外显子I编码mRNA的5'-未翻译区。人类和大鼠CRH基因的核苷酸序列同源性分析揭示了CRH肽编码序列和5'侧链序列等高度保守区域。RNA印迹分析表明，CRH mRNA可以在大鼠大脑的许多区域以及脊髓、肾上腺、垂体和睾丸中观察到。
The rat corticotropin releasing hormone (CRH) gene has been isolated and characterized by DNA sequence analysis. The gene exhibits a structural organization similar to that of the human CRH gene. The nucleotide sequence encoding the entire rat CRH precursor is located on the second exon, while exon I encodes the 5'-untranslated region of the mRNA. Analysis of the nucleotide sequence homology between the human and rat CRH genes reveals several highly conserved regions including the CRH peptide-encoding sequence and the 5'-flanking sequence. RNA blot analysis demonstrates that CRH mRNA can be observed in numerous regions of the rat brain as well as the spinal cord, adrenal gland, pituitary, and testis.