Mouse submandibular gland mucin: embryo-specific mRNA and protein species
Mouse submandibular gland mucin: embryo-specific mRNA and protein species
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DOI：
10.1016/s0925-4773(98)00062-8
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发表时间：
1998-06-01
影响因子：
2.6
通讯作者：
Melnick, M
中科院分区：
文献类型：
--
作者：
Jaskoll, T;Chen, HM;Melnick, M
小鼠颌下腺(SMG)粘蛋白是颌下上皮细胞的主要组织分化产物。通过Northern和Western印迹分析，我们证明了胚胎、新生儿和成人SMG粘蛋白mRNA和蛋白的显著差异：E17和1天大的新生儿表现出两个独特的粘蛋白转录本(1.20和0.85kb)，其大小大约比单个成人转录本(1.01kb)大或小19%。免疫检测到两种胚胎蛋白亚型(M(R)类似于110和152 kDa)，与单个成体蛋白(M(R)类似于136 kDa)相比，较大的(类似152 kDa)胚胎亚型持续存在于新生儿腺体中。在E14和老年SMG中，粘蛋白转录定位于分支上皮细胞，随着年龄的增长，顶芽和腺泡前上皮细胞中的杂交信号增加；通过RNase保护分析，在E14到E19之间，转录水平显著增加26%。相反，下颌下粘蛋白直到胚胎17岁才被免疫检测到，主要免疫定位于顶芽和腺泡旁上皮细胞膜。我们的数据清楚地表明，胚胎和成体SMG粘蛋白的mRNA和蛋白质之间存在实质性的质量差异。(C)1998爱思唯尔爱尔兰科学有限公司。保留所有权利。
Mouse submandibular salivary gland (SMG) mucin is the primary histodifferentiation product of submandibular epithelia. We demonstrate marked differences between embryonic, neonatal, and adult SMG mucin mRNA and protein by Northern and Western blot analyses: E17 and 1-day-old neonates exhibit two unique mucin transcripts (1.20 and 0.85 kb) which are approximately 19% greater or smaller in size than the single (1.01 kb) adult transcript. Two embryonic protein isoforms (M(r)similar to 110 and 152 kDa) are immunodetected compared to a single adult protein (M(r)similar to 136 kDa), with the larger (similar to 152 kDa) embryonic isoform persisting in neonatal glands. Mucin transcripts are localized to the branching epithelia in E14 and older SMGs, with increased hybridization signal being seen in terminal bud and proacinar epithelial cells with age; a significant 26% increase in transcript levels is detected by RNase protection assay between E14 and E19. By contrast, submandibular mucin protein is not immunodetected until E17, being primarily immunolocalized to terminal bud and proacinar epithelial cell membranes. Our data clearly shows that substantial qualitative differences exist between embryonic and adult SMG mucin mRNA and protein. (C) 1998 Elsevier Science ireland Ltd. All rights reserved.