Acute bladder decentralization in hound dogs: Preliminary results of effects on hypogastric nerve electroneurograms and detrusor pressure responses to spinal root and hypogastric nerve stimulation
Acute bladder decentralization in hound dogs: Preliminary results of effects on hypogastric nerve electroneurograms and detrusor pressure responses to spinal root and hypogastric nerve stimulation
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DOI：
10.1371/journal.pone.0215036
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发表时间：
2019-04-10
期刊：
影响因子：
3.7
通讯作者：
Ruggieri, Michael R., Sr.
中科院分区：
文献类型：
--
作者：
Tiwari, Ekta;Barbe, Mary F.;Ruggieri, Michael R., Sr.
目的改进膀胱充盈状态下神经电活动监测技术，检测正常完整膀胱和急性分散膀胱的神经活动。方法观察电刺激下神经或腰前根对逼尿肌压力的影响，以及异氟醚和异丙酚麻醉对下腹部神经刺激诱发压力的影响。然后在脊髓和电极之间的膀胱充盈前后使用定制的双极袖带电极记录下腹下神经的活动，以消除传出神经信号。结果电刺激下腹下神经引起的低幅度逼尿肌压力在两种麻醉剂之间没有差异。上腰(L2)腹根刺激引起的逼尿肌压力在切断腹下神经和L5以下的所有脊神经后受到抑制，但并未消除。在神经完整的膀胱中，腹下神经的传入和传出活动不随膀胱充盈而改变，而在分散的膀胱中，传入和传出神经的活性降低。在完整或分散膀胱充盈过程中，未观察到传入活动的变化。结论更完整的分散性膀胱模型应包括横断支配膀胱的腰骶脊神经根和腹下神经。这些改进的神经电信号记录方法可能适用于通过监测神经移位神经的感觉活动来评估神经移位手术治疗膀胱神经再支配的效果。
ObjectiveWe aimed to refine electroneurogram techniques for monitoring hypogastric nerve activity during bladder filling, and then examined nerve activity in normal intact versus acutely decentralized bladders.MethodsEffects of electrical stimulation of hypogastric nerves or lumbar ventral roots on detrusor pressure were examined, as were effects of isoflurane versus propofol anesthetics on hypogastric nerve stimulation evoked pressure. Hypogastric nerve activity was then recorded using custom-made bipolar cuff electrodes during bladder filling before and after its transection between the spinal cord and electrode to eliminate efferent nerve signals.ResultsElectrical stimulation of hypogastric nerves evoked low amplitude detrusor pressures that did not differ between the two anesthetics. Upper lumbar (L2) ventral root stimulation evoked detrusor pressures were suppressed, yet not eliminated, after transection of hypogastric nerves and all spinal roots below L5. Afferent and efferent hypogastric nerve activity did not change with bladder filling in neuronally intact bladders yet decreased in decentralized bladders. No change in afferent activity was observed during bladder filling in either intact or decentralized bladders.ConclusionsThese findings indicate that a more complete decentralized bladder model should include transection of lumbosacral spinal roots innervating the bladder as well as hypogastric nerves. These refined electroneurogram recording methods may be suitable for evaluating the effectiveness of nerve transfer surgeries for bladder reinnervation by monitoring sensory activity in the transferred nerve.