Cloning of GPR37, a gene located on chromosome 7 encoding a putative G-protein-coupled peptide receptor, from a human frontal brain EST library
Cloning of GPR37, a gene located on chromosome 7 encoding a putative G-protein-coupled peptide receptor, from a human frontal brain EST library
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DOI：
10.1006/geno.1997.4900
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发表时间：
1997-10-01
期刊：
影响因子：
4.4
通讯作者：
TocchiniValentini, GP
中科院分区：
文献类型：
--
作者：
Marazziti, D;Golini, E;TocchiniValentini, GP
从人脑额叶表达序列标签中克隆了一段编码肽特异性G蛋白偶联受体（GPR 37）的cDNA序列。GPR 37 cDNA预测了一个单一的开放阅读框架，编码一个613个氨基酸的蛋白质，具有7个疏水跨膜结构域。将GPR 37基因组序列定位于染色体7 q31，并在筛选染色体7特异性基因组文库时分离。GPR 37基因跨度超过25 kb，包含两个外显子和一个内含子，该内含子在编码推定的第三跨膜结构域的序列内中断GPR 37 cDNA。用GPR 37探针进行的北方印迹分析显示主要的3.8 kb mRNA和较少的8 kb mRNA，两者都在人脑组织中表达，特别是在胼胝体、髓质、壳核和尾状核中。小脑中表达水平最低。3.8kb的mRNA在肝脏和胎盘中的表达量也较低。虽然尚未鉴定出推定的GPR 37受体的配体，但其推导的氨基酸序列显示出与大多数哺乳动物肽特异性G蛋白偶联受体，特别是与人内皮素-B、铃蟾肽-BB 1和铃蟾肽-BB 2受体的高度同源性（在跨膜区中类似于40）。(C)北京：科学出版社.
A cDNA sequence encoding a putative peptide specific G-protein-coupled receptor (GPR37) was isolated from a set of human brain frontal lobe expressed sequence tags. The GPR37 cDNA predicts a single open reading frame coding for a 613-amino-acid protein with seven hydrophobic transmembrane domains. The GPR37 genomic sequence was mapped to chromosome 7q31, and it was isolated upon screening of a chromosome 7-specific genomic library. The GPR37 gene spans more than 25 kb and contains two exons and a single intron which interrupts the GPR37 cDNA within the sequence encoding the presumed third transmembrane domain. Northern blot analysis with GPR37 probes revealed a main 3.8-kb mRNA and a less abundant 8-kb mRNA, both expressed in human brain tissues, particularly in corpus callosum, medulla, putamen, and caudate nucleus. The lowest level of expression was detected in cerebellum. The 3.8-kb mRNA is also less abundantly expressed in liver and placenta. Although the ligand for the putative GPR37 receptor has not been identified, its deduced amino acid sequence shows a high degree of homology (similar to 40 in the transmembrane regions) with most mammalian peptide-specific G-protein-coupled receptors and particularly with the human endothelin-B, bombesin-BB1, and bombesin-BB2 receptors. (C) 1997 Academic Press.