Real‐Time Chemiluminescence Imaging Using Nano‐Lantern Probes
Real‐Time Chemiluminescence Imaging Using Nano‐Lantern Probes
复制标题
使用纳米灯笼探针进行实时化学发光成像
DOI：
10.1002/9780470559277.ch140168
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发表时间：
2014
影响因子：
--
通讯作者：
T. Nagai
中科院分区：
文献类型：
--
作者：
Y. Arai;T. Nagai
化学发光成像可以在没有激发光源的情况下在从单个细胞到整个身体的各种空间水平上进行。到目前为止，化学发光成像主要是在较长的曝光时间下进行的，因为信号弱，导致时间分辨率低。最近，已知最亮的化学发光蛋白——纳米灯和基于纳米灯的功能指示物——已经被开发出来。纳米灯探针打破了时间分辨率的限制，能够以视频速率对细胞、植物和小动物等活体样品进行化学发光成像。本单元描述了一种用于观察自由移动的未剃毛小鼠移植表达纳米灯笼的肿瘤细胞的方案，以及另一种用于兼容使用光遗传学工具和纳米灯笼钙指示剂的方案。这两种方案都利用了照明和相机采集会话的同步，从而实现了实时化学发光成像。©2014 by John Wiley & Sons, Inc。
Chemiluminescence imaging can be performed without excitation light sources at various spatial levels ranging from a single cell to the whole body. Thus far, chemiluminescence imaging has been primarily performed with long exposure times because of weak signals, resulting in low temporal resolution. Recently, the brightest‐known chemiluminescent proteins—Nano‐lantern and Nano‐lantern‐based functional indicators—have been developed. Nano‐lantern probes break the limitation of temporal resolution and enable chemiluminescence imaging of living samples such as cells, plants, and small animals at video rates. This unit describes one protocol for observation of a freely moving unshaved mouse transplanted with Nano‐lantern‐expressing tumor cells, and another for compatible use of optogenetic tools and a Nano‐lantern calcium indicator. Both protocols utilize the synchronization of illumination and camera acquisition sessions, thereby enabling real‐time chemiluminescence imaging. © 2014 by John Wiley & Sons, Inc.