Proteome changes in human bronchoalveolar cells following styrene exposure indicate involvement of oxidative stress in the molecular-response mechanism.
Proteome changes in human bronchoalveolar cells following styrene exposure indicate involvement of oxidative stress in the molecular-response mechanism.
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DOI：
10.1002/pmic.200800836
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发表时间：
2009-11
期刊：
影响因子：
3.4
通讯作者：
von Bergen, Martin
中科院分区：
文献类型：
--
作者：
Moerbt, Nora;Moegel, Iljana;Kalkhof, Stefan;Feltens, Ralph;Roeder-Stolinski, Carmen;Zheng, Jiang;Vogt, Carsten;Lehmann, Irina;von Bergen, Martin
关键词：
苯乙烯是一种挥发性有机化合物（VOC），广泛用作许多工业环境中的中间体。在环境显著浓度下，已知对健康有不利影响，但对接触亚急性毒性浓度的苯乙烯的分子效应知之甚少。我们将人肺上皮细胞在宽浓度范围（1 mg/m3-10 g/m3）下暴露于苯乙烯，并通过2-DE分析对蛋白质组水平的影响，其中检测到1，380个蛋白质点，并通过质谱法明确鉴定了266个。发现一组16个蛋白质斑点由于暴露于环境显著浓度为1-10 mg/m3（0.2 - 2.3 ppm）的苯乙烯而发生显著改变。其中，超氧化物歧化酶[Cu-Zn]以及胆绿素还原酶A可能与氧化应激的分子途径相关，而真核生物翻译起始因子5A-1、ezrin、核纤层蛋白B2和电压依赖性阴离子通道2已被报道参与细胞凋亡。用苯乙烯处理也引起苯乙烯氧化物-蛋白质加合物的形成，特别是硫氧还蛋白还原酶1。这些结果强调了相关的氧化应激作为一个主要的分子反应机制的肺上皮细胞在室内相关浓度的苯乙烯暴露。
Styrene is a volatile organic compound (VOC) that is widely used as an intermediate in many industrial settings. There are known adverse health effects at environmentally significant concentrations, but little is known about the molecular effect of exposure to styrene at subacute toxic concentrations. We exposed human lung epithelial cells, at a wide range of concentrations (1 mg/m3-10 g/m3), to styrene and analyzed the effects on the proteome level by 2-DE, where 1,380 proteins spots were detected and 266 were identified unambiguously by mass spectrometry. A set of 16 protein spots was found to be significantly altered due to exposure to styrene at environmentally significant concentrations of 1-10 mg/m3 (0.2 – 2.3 ppm). Among these, superoxide dismutase [Cu-Zn] as well as biliverdin reductase A could be correlated with the molecular pathway of oxidative stress, while eukaryotic translation initiation factor 5A-1, ezrin, lamin B2 and voltage dependent anion channel 2 have been reported to be involved in apoptosis. Treatment with styrene also caused formation of styrene oxide-protein adducts, specifically for thioredoxin reductase 1. These results underline the relevance of oxidative stress as a primary molecular response mechanism of lung epithelial cells to styrene exposure at indoor-relevant concentrations.
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影响因子：
4.8
作者：
Dammeyer, Pascal;Damdimopoulos, Anastasios E.;Arner, Elias S. J.
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作者：
Benndorf, Dirk;Balcke, Gerd U.;von Bergen, Martin
通讯作者：
von Bergen, Martin