Heparin-induced circular dichroism of chloroquine.
Heparin-induced circular dichroism of chloroquine.
复制标题
肝素诱导的氯喹圆二色性。
DOI：
10.1016/j.bbrc.2009.07.098
复制
发表时间：
2009
影响因子：
3.1
通讯作者：
Stalcup,AM
中科院分区：
文献类型：
--
作者：
Stanley,FE;Warner,AM;Gutierrez,SM;Stalcup,AM
圆二色性（CD）和紫外/可见吸收（UV/维斯）光谱技术被用来研究肝素和氯喹之间的相互作用，氯喹是一种抗疟疾药物，已显示出作为抗朊病毒剂的潜力。加入肝素后外消旋氯喹的CD光谱提供了糖胺聚糖（GAG）结合的证据，支持最近的研究结果表明肝素和抗疟药物之间的相互作用主要是由于静电相互作用，并代表了首次报道的GAG诱导的双环芳香族化合物的CD信号。氯喹和肝素之间的缔合常数（Δ 103 M −1）是根据紫外滴定曲线计算的，并提供了对这两种化合物之间缔合性质的进一步了解。
Circular dichroism (CD) and UV/Visible absorption (UV/Vis) spectroscopy techniques were used to investigate the interaction between heparin and chloroquine, an antimalarial drug that has shown potential as an anti-prion agent. CD spectra of rac-chloroquine upon addition of heparin provide evidence of glycosaminoglycan (GAG) binding, support recent findings suggesting that interactions between heparin and antimalarial drugs are largely due to electrostatic interactions, and represent the first reported GAG-induced CD signal of a bicyclic, aromatic compound. The association constant (∼103M−1) between chloroquine and heparin was calculated from a UV titration curve and provided additional insight into the nature of the association between these two compounds.