Development of a method to rapidly assess resistance/susceptibility of Micro-Tom tomatoes to Tomato yellow leaf curl virus via agroinoculation of cotyledons.
Development of a method to rapidly assess resistance/susceptibility of Micro-Tom tomatoes to Tomato yellow leaf curl virus via agroinoculation of cotyledons.
复制标题
通过子叶农业接种快速评估Micro-Tom番茄对番茄黄化曲叶病毒的抗性/敏感性的方法的开发。
DOI：
10.1186/s13104-021-05651-3
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发表时间：
2021-06-23
影响因子：
1.8
通讯作者：
Sera T
中科院分区：
文献类型：
--
作者：
Mori T;Takenaka K;Domoto F;Aoyama Y;Sera T
番茄黄化曲叶病毒(TYLCV)是严重危害番茄作物的病原之一。因此，需要开发治疗或预防TYLCV感染的方法。为此目的，需要一种方便、快速地评估番茄TYLCV侵染的方法。在本研究中，我们建立了一种利用微型番茄品种Micro-Tom的子叶来快速评估TYLCV侵染的方法。首先，我们构建了含有1.5个拷贝的TYLCV基因组的双元质粒，并将其转化农杆菌。通过将转化子中的农杆菌注入Micro-TOM的枝条中，我们证实了Micro-TOM对TYLCV的敏感性。为了进一步缩短TYLCV侵染的评估过程，我们在播种后10天对Micro-Tom的子叶进行了接种。接种10天后，我们在真叶上连续观察到TYLCV感染的典型症状。分子检测结果显示，接种后6天，所有表现症状的叶片中都检测到了TYLCV后代DNA。因此，我们的新方案能够在播种后20天内评估TYLCV感染。因此，接种小番茄子叶将加快筛选抗TYLCV的小番茄的进程，并能更快地筛选出更多的植株，有助于发展抗TYLCV的番茄。网上版载有补充材料，可在10.1186/s13104021-05651-3查阅。
Tomato yellow leaf curl virus (TYLCV) is one of the pathogens severely damaging tomato crops. Therefore, methods to treat or prevent TYLCV infection need to be developed. For this purpose, a method to conveniently and quickly assess infection of tomatoes by TYLCV is desired. In the present study, we established a quick method to evaluate TYLCV infection using cotyledons of Micro-Tom, a miniature tomato cultivar. First, we constructed a binary plasmid harboring 1.5 copies of the TYLCV genome and transformed Agrobacterium with the plasmid. By injecting agroinoculum from the resulting transformant into the branches of Micro-Tom, we confirmed the susceptibility of Micro-Tom to TYLCV. To shorten the evaluation process of TYLCV infection further, we agroinoculated cotyledons of Micro-Tom 10 days after sowing seeds. We consistently observed typical symptoms of TYLCV infection on true leaves 10 days after agroinoculation. Molecular analysis detected TYLCV progeny DNA in all leaves demonstrating symptoms 6 days after agroinoculation. Therefore, our new protocol enabled assessment of TYLCV infection within 20 days after sowing seeds. Thus, agroinoculation of Micro-Tom cotyledons will accelerate the process of screening TYLCV-resistant Micro-Toms and enable screening of larger numbers of plants more quickly, contributing to the development of TYLCV-resistant tomatoes. The online version contains supplementary material available at 10.1186/s13104-021-05651-3.