Rice PPS1 encodes a DYW motif-containing pentatricopeptide repeat protein required for five consecutive RNA-editing sites of nad3 in mitochondria
Rice PPS1 encodes a DYW motif-containing pentatricopeptide repeat protein required for five consecutive RNA-editing sites of nad3 in mitochondria
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水稻 PPS1 编码线粒体中 nad3 五个连续 RNA 编辑位点所需的包含 DYW 基序的五肽重复蛋白
DOI：
10.1111/nph.15347
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发表时间：
2018
期刊：
影响因子：
9.4
通讯作者：
Hu Jun
中科院分区：
文献类型：
--
作者：
Xiao Haijun;Zhang Qiannan;Qin Xiaojian;Xu Yanghong;Ni Chenzi;Huang Jishuai;Zhu Linlin;Zhong Feiya;Liu Wei;Yao Guoxin;Zhu Yingguo;Hu Jun
五肽重复（PPR）蛋白家族是一个由退化的35个氨基酸基序串联而成的大家族，其成员在转录后水平上作为细胞器基因表达的重要调节因子。尽管PPRs在细胞器中的RNA编辑中发挥作用，但其编辑活性及其潜在机制尚不清楚。在这里，我们发现一个新的含有DYW基序的PPR蛋白PPS1与nad3的5个保守的RNA编辑位点相关，这些位点位于线粒体中彼此靠近，所有这些位点都涉及水稻从脯氨酸到亮氨酸的转化。thpps1rnai和杂合植物在营养期和生殖期都具有发育迟缓和花粉部分不育的特点。RNA电泳迁移迁移分析（remsa）和使用不同版本nad3探针的互反竞争分析证实，PPS1可以结合五个受影响位点附近的顺式元件，这与现有的PPR - RNA结合模式不同，因为编辑位点是连续性的。编辑atnad3inpps1的缺失会降低线粒体电子传递链中几种复合物的活性，并影响线粒体形态。综上所述，我们的研究结果表明pps1是水稻中特定编辑位点所必需的。
The pentatricopeptide repeat (PPR) protein family is a large family characterized by tandem arrays of a degenerate 35‐amino‐acid motif whose members function as important regulators of organelle gene expression at the post‐transcriptional level. Despite the roles of PPRs in RNA editing in organelles, their editing activities and the underlying mechanism remain obscure.Here, we show that a novel DYW motif‐containing PPR protein, PPS1, is associated with five conserved RNA‐editing sites ofnad3located in close proximity to each other in mitochondria, all of which involve conversion from proline to leucine in rice. Bothpps1RNAi and heterozygous plants are characterized by delayed development and partial pollen sterility at vegetative stages and reproductive stage.RNA electrophoresis mobility shift assays (REMSAs) and reciprocal competition assays using different versions ofnad3probes confirm that PPS1 can bind to cis‐elements near the five affected sites, which is distinct from the existing mode of PPR‐RNA binding because of the continuity of the editing sites. Loss of editing atnad3inpps1reduces the activity of several complexes in the mitochondrial electron transport chain and affects mitochondrial morphology.Taken together, our results indicate thatPPS1is required for specific editing sites innad3in rice.